World Journal of Cancer Research 世界肿瘤研究, 2024, 9(3), 82-89
Published Online July 2024 in Hans. http://www.hanspub.org/journal/wjcr https://doi.org/10.12677/wjcr.2024.93012
The Mechanism of TMEM176B in Apoptosis of Breast Cancer
Chong Yang1, Baochang Su2*
12
Guangzhou Health Science College, Guangzhou Guangdong
Department of Blood Transfusion, The First Affiliated Hospital of Jinan University, Guangzhou Guangdong
ththth
Received: Jun. 26, 2024; accepted: Jul. 18, 2024; published: Jul. 25, 2024
Abstract
Objective: To study the role and mechanism of transmembrane protein 176B (TMEM176B) in breast cancer. Methods: The expression of TMEM176B in breast cancer tissue was detected by qRT-PCR and Western blot, and using small interfering RNA for silencing TMEM176B level. MTT assay and CCK8 assay were used to detect the viability of breast cancer cells, and flow cytometry was used to detect the apoptosis of breast cancer cells. Results: Small interfering RNA significantly knocked down the level of TMEM176B, inhibited the growth of breast cancer. In terms of mechanism, siRNA inhibited the cell proliferation protein Ki67 expression, and down-regulated the expression of apoptotic protein Bcl-2 and up-regulated the expression of apoptotic protein Bax. Furthermore, the result showed that the expression of p53 and the level of p-AKT were down-regulated after TMEM176B interference. Conclusion: knockdown of TMEM176B promotes the apoptosis of breast cancer by regulating the expression of p53 and the level of p-AKT.
Keywords
Breast Cancer, TMEM176B, Bcl-2, Bax, Ki67, P53 and P-AKT Signaling Pathways
TMEM176B调控乳腺癌凋亡作用及机制研究
杨 翀1，苏宝倡2*
12
收稿日期：2024年6月26日；录用日期：2024年7月18日；发布日期：2024年7月25日
*广州卫生职业技术学院，广东 广州 暨南大学附属华侨医院，广东 广州
通讯作者。
文章引用: 杨翀, 苏宝倡. TMEM176B调控乳腺癌凋亡作用及机制研究[J]. 世界肿瘤研究, 2024, 9(3): 82-89. DOI: 10.12677/wjcr.2024.93012
TMEM176B调控乳腺癌凋亡作用及机制研究
