DETERMINING DEVICE FOR APPANGEMENT OF DNA
BASE
申请(专利)号: JP19930264320
专利号: JPH0798276A 主分类号: G01N21/64 申请权利人: HITACHI
ELECTRON ENG CO LTD 公开国代码: JP 优先权国家: JP
摘 要:
PURPOSE: To make it possible to detect fluorescence efficiently without the adverse effect of smiling by using a
fluorescence detecting means comprising a refractive-index distribution type lens array filter and a charge-coupled-device line sensor. ;CONSTITUTION: When laser light 90 is cast on a DNA cut piece 76, which is migrated along each
migrating path 88 of a gel electrolyte layer 86 of a migrating plate 74 in the lateral direction, fluorescence is emitted. The fluorescence is cast into a refractive-index-distribution type lens array 3. The passed light is cast into a filter 5. The stray light, background light or the like having the wavelength other than the fluorescence component is cut.
Thereafter, the fluorescence, which has passed through the filter 5, is received with a CCD line sensor 7 and converted into the electric signal. Even if the smiling occurs in the sample during the migration,
申请日: 1993-09-28 公开公告日: 1995-04-11
分类号: G01N21/64;
G01N27/447 发明设计人: MISHINA
YOSHINORI; NEMOTO RYOJI 申请国代码: JP
优先权: 19930928 JP
26432093
摘 要 附 图:
the fluorescence can be effectively
detected by the use of this fluorescence detecting means 1. When the refractive-index- distribution type lenses are alinged in the array state, the neighboring lens images are overlapped, and the errecting-image focusing system is obtained. The interference of the image focusing system between the neighboring migrating paths do not occur.;COPYRIGHT: (C)1995,JPO 主权项:
【請求項1】 DNA断片用の多数の泳動路を有する、垂直に保持される平板型ゲル電気泳動手段、該電気泳動装置の泳動路に、該電気泳動装置の横方向から該泳動路と直交するように、レーザ光を照射する光励起用のレ
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