Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance

Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect

resistance

佚名

【期刊名称】《自然科学进展（英文版）》 【年(卷),期】2002(012)003

【摘要】The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence (tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco. 【总页数】6页(198-203)

【关键词】tomato proteinase inhibitor II gene;transgenic tobacco;insect-resistance 【作者】佚名