Protective effects of organic extracts of Alpinia oxyphylla against hydrogen peroxide-indu

Protective effects of organic extracts of Alpinia oxyphylla against hydrogen peroxide-induced

cytotoxicity in PC12 cells

Li-Hong Duan1, 2, 3, 4, 5, #, Meng Li1, #, Chun-Bao Wang1, 2, 5, 6, 7, 8, ＊, Qing-Mei Wang3, Quan-Quan Liu1, 2, 5, 8, Wan-Feng Shang1, 2, 5, Ya-Jin Shen4,Zhuo-Hua Lin8, Tong-Yang Sun6, Zheng-Zhi Wu1, 2, 5, ＊, Ying-Hong Li1, 2, 5, Yu-Long Wang2, 5, Xun Luo9 【摘 要】Abstract

【期刊名称】《中国神经再生研究（英文版）》 【年(卷),期】2020(015)004 【总页数】8

【关键词】Key Words: active ingredients; Alpinia oxyphylla; apoptosis; ethanol crude extract; fraction; hydrogen peroxide; nerve regeneration; neuroprotective agent; neuroprotective effects; PC12 cells; traditional herb

RESEARCH ARTICLE

Funding: This work was financially supported by the National Natural Science Foundation of China, No. 81574038 (to ZZW); the Natural Science

Foundation

of

Guangdong

Province

of

China,

No.

2017A030313842 (to LHD); the Science and Technology Foundation of Guangdong Province of China, No. 2017A050506007 (to YHL); the Technology Research Foundation of Basic Research Project of Shenzhen

City of China,No. JCYJ20170412161254416 (to ZZW). Graphical Abstract ＊

Correspondence

to:Chun-Bao

Wang, Wu,

PhD,chunbaowang@szu.edu.cn;Zheng-Zhi PhD,szwzz001@szu.edu.cn.

#Both authors contributed equally to this study. orcid:0000-0002-8795-0142(Chun-Bao 609X(Zheng-Zhi Wu) Received: May 20, 2019

Peer review started: May 22, 2019 Accepted: August 14, 2019

Published online: October 15, 2019

Chinese Library Classification No. R452; R363; R364

Wang)0000-0002-8819-

Introduction

Alpinia (A.) oxyphylla is commonly used in traditional Chinese medicine to treat dyspepsia, diarrhea (Zhang et al.,2013; Wang et al., 2015), abdominal pain (Song et al., 2014;Zhang et al., 2015a), poor memory (Shi et al., 2015; He et al.,2019), inflammatory conditions (He et al., 2010; Zhang et al.,2018; Qi et al., 2019) and cancer (Lin et al., 2013). Recently,the medicinal properties of A. oxyphylla and its pharmaceutical products have received considerable attention (Bian et al., 2013; Zhang et al., 2015b, 2018). The protective effects of A. oxyphylla extract in

chronic kidney disease have been explored using metabolomics (Li et al., 2016). Various biomarkers, such as agmatine, CAMP and 7-methylguanine,are restored to control levels after treatment with A. oxyphylla extract, suggesting that it has protective effects. Wang et al. (2015) showed that the 95% ethanol extract and 90%ethanol-eluted fractions of A. oxyphylla have antidiarrheal activity. Some studies focusing on the effectiveness of A.oxyphylla extract ignored identifying the bioactive components. A. oxyphylla contains numerous potentially bioactive compounds, including flavonoids (Zhang et al., 2015b; Sun et al., 2016), tepenes (Lv et al., 2011; Xie et al., 2014; Hou et al., 2015; Zhao et al., 2015), alkaloids (Zhou et al., 2013) and diphenylheptanes (Bian et al., 2013).

Peripheral nerve injuries, such as those caused by accidental trauma, birth injury, ischemia or iatrogenic injury, often result in temporary or life-long neurological dysfunctions,which can be devastating and severely impact the patient’s quality of life (Cao et al., 2019; Han et al., 2019; Zhang et al., 2019). It is necessary to promote neural cell proliferation to restore the injured nerves in adults. A few studies have shown that protocatechuic acid modulates the MAPK(ERK1/2, JNK and p38)/PA (uPA, tPA)/MMP (MMP2,MMP9) regeneration and migration signaling pathways in Schwann cells (Ju et al., 2015a). Furthermore, protocatechuic acid promotes cell proliferation and survival via the