Sperm immobilization activity of Allium sativum L. and other plant extracts - 鐧惧害鏂囧簱

Sperm immobilization activity of Allium sativum L.

and other plant extracts

KausikiChakrabarti;SulagnaPal;AsokK.Bhattacharyya

【期刊名称】《亚洲男性学杂志：英文版》 【年(卷),期】2003(005)002

【摘要】Aim: To identify possible spermicidal agents through screening a number of edible medicinal plants with antimicrobial activity. Methods: Initial screening was made on the basis of ram cauda epididymal sperm immobiliza-tion immediately after addition of extracts. The most potent extract was selected and was evaluated on both ram and human spermatozoa. To unravel its mode of action several sperm functional tests were carried out, namely viability of cells, hypo-osmotic swelling test for membrane integrity and assays of membrane-bound enzyme 5'-nucleotidase and acrosomal marker enzyme acrosin. Results: The crude aqueous extract of the bulb ofAllium sativum L. Showed the most promising results by instant immobilization of the ram epididymal sperm at 0.25 g/mL and human ejaculated sperm at 0.5 g/mL. Sperm immobilizing effects were irreversible and the factor of the extract responsible for immobilization was thermostable up to 90℃. On boiling at 100℃ for 10 minutes, this activity was markedly reduced. Moreover, this extract was able to cause aggregation of ram sperms into small clusters after 30 minutes of incubation at 37℃. However this property