HPLC 法测定六白白疕巴布剂中血竭素的含量
张伟;李静;郭雪申;王超;于爽;孙晶
【期刊名称】《光明中医》 【年(卷),期】2015(000)004
【摘要】目的：建立六白白疕巴布剂中血竭素的含量测定方法。方法采用HPLC法，以乙腈－0．05 mol?L－1磷酸二氢钠溶液（50∶50）为流动相，流速1 mL?min－1，检测波长440 nm，柱温40℃，测定六白白疕巴布剂中血竭素的含量。结果血竭素在1．923～19．23μg?mL－1范围内呈良好的线性关系（r＝0．9974），平均加样回收率为97．85％，RSD为0．89％（n＝5）。结论此法简便、准确、专属性强。%Objective To established the HPLC method to determine dracorhodin in Liubai Baibi cataplasm.Method We took the HPLC method,with a mobile phase of acetonitrile-0.05mol?L-1 NaH2PO4(50∶50), flowing rates: 1.0 ml? min-1, and detecting wavelength:440nm, column temperature:40℃.Results The sample size of dracorhodin in the range of 1.923~19.23μg?mL-1 integral value of peak area was linear relationship(r=0.9974).The average recovery was 97.85%and RSD was 0.89%(n=5).Conclusion The method was simple,rapid,accurate and specific. 【总页数】3页(728-730)
【关键词】六白白疕巴布剂;血竭素;HPLC 【作者】张伟;李静;郭雪申;王超;于爽;孙晶
【作者单位】山东省青岛市海慈医疗集团制剂实验室 青岛 266033;山东省青岛
HPLC 法测定六白白疕巴布剂中血竭素的含量
