常见恶性肿瘤端粒酶活性检测
刘晓军;邹雅群;郭辰虹;陈丙玺;卢勇;龚瑶琴
【期刊名称】《中华肿瘤杂志》 【年(卷),期】2001(023)001
【摘要】Objective To study the usefulness of detection of telomerase activity in the diagnosis of malignant tumors. Methods Modified telomeric repeat amplification protocol (TRAP) was employed to examine telomerase activity in 283 malignant tumor tissuses, 42 adjacent tissuses and 61 benign tumor tissuses. Results Of the 283 malignant tumors and 42 adjacent tissues, telomerase was positive in 264 (93.3%) and 11 (26.1%), respectively. Only 4 of the 62 (6.6%) benign tumors were telomerase positive. Conclusion Telomerase activity may be used as tumor marker in the diagnosis of malignant tumors.%目的探讨端粒酶活性检测在常见恶性肿瘤诊断中的作用。方法采用改良的银染端粒重复序列扩增方法，对283例恶性肿瘤、61例良性肿瘤和42例癌旁组织进行端粒酶活性检测。结果 283例恶性肿瘤中，264例检测到端粒酶活性，阳性率为93.3%；61例良性肿瘤中，4例检测到端粒酶活性，阳性率为6.6%；42例癌旁组织中，11例检测到端粒酶活性，阳性率为26.2%。结论端粒酶的激活与恶性肿瘤的发生发展密切相关。端粒酶可以作为一种有效的恶性肿瘤标记物用于恶性肿瘤的诊断。 【总页数】3页(25-27)
【关键词】肿瘤/诊断;端粒酶/分析
常见恶性肿瘤端粒酶活性检测
