水杨酸调节决明根系铝诱导的氧化胁迫
王猷生;汪瑾;杨志敏;王庆亚;吕波;李少琼;卢亚萍;王松华;孙新
【期刊名称】《植物学报（英文版）》 【年(卷),期】2004(046)007
【摘要】Salicylic acid (SA) plays an important role in mediating some biotic and abiotic stress-induced oxidative stresses in plants. However, it remains unknown about the role of SA in mediating oxidative stress induced by aluminum. In this study, we investigated the changes in concentrations of H2O2 and O2-,some antioxidative enzyme activities and several physiological parameters involved in oxidative damage to plasma membrane in the root tips of Cassia tora L. Results indicated that 20 μmol/L aluminum (Al) caused increases in electrolytes leakage, malondlaldehyde (MDA) content and intense staining with Evans blue in root tips, while treatment with 5 μmol/L SA suppressed the Al-induced increase in MDA. Examination of H2O2 and O2-, the major ROS responsible for lipid peroxidation indicated that root tips challenged with Al in the presence of SA contained significantly lower contents of H2O2 and O2-than those with Al alone.However, the reduced levels of H2O2 appeared not to be correlated with enhanced activities of ROSscavenging enzymes like catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR), but with increased activities of guaiacol peroxidase (POD). SA-induced reduction of H2O2 was also correlated
with suppression of O2-accumulation and superoxide dismutase (SOD) activities. From the results, it is concluded that SA might activate an H2O2-mediated pathway, which in turn initiated a POD-dependent antioxidative mechanism for retarding lipid peroxidation or preserving membrane integrity in root tips of C. tora. We presented the evidence of time-dependent changes in endogenous SA in root tips exposed to 20 μmol/L Al.%水杨酸(Salicylic acid,SA)在调节生物和非生物胁迫,诱导植物氧化胁迫中起着重要的作用,但对铝诱导的氧化胁迫的调节作用尚不清楚.本文研究了SA对决明(Cassiatora L.)根系铝诱导的H2O2和O2-含量变化,包括抗氧化酶活性以及细胞质膜过氧化胁迫变化的影响.介质中20 μmol/L铝处理增加质膜透性,导致MDA含量上升及根尖细胞Evans blue染色加重(测定细胞死亡),而外源供给5 μmol/L SA能缓解铝诱导的氧化胁迫.SA处理能明显降低根尖H2O2和O2-的含量,但两者含量与CAT、APX和GR的活性变化没有相关性,而与POD活性增加有关.水杨酸诱导H2O2含量的下降与抑制O2积累和SOD活性有关.结果表明,SA可能激活一条由H2O2介导的、依赖于POD的抗氧化机制来缓解脂质的过氧化作用. 【总页数】10页(819-828)
【关键词】水杨酸;铝毒;氧化胁迫;过氧化氢;决明(Cassia tora) 【作者】王猷生;汪瑾;杨志敏;王庆亚;吕波;李少琼;卢亚萍;王松华;孙新
【作者单位】南京农业大学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大
学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大学生命科学学院生物化学与分子生物学系,南京,210095;南京农业大学生命科学学院生物化学与分子生物学系,南京,210095 【正文语种】中文 【中图分类】Q945 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_journal-integrative-plant-biology_thesis/0201255534903.html 【相关文献】
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水杨酸调节决明根系铝诱导的氧化胁迫
