Matrigel胶对高氧损伤早产鼠肺泡Ⅱ型细胞黏着斑激酶表
达变化及其对肺泡Ⅱ型细胞增殖和凋亡的影响
王华;李文斌;常立文
【期刊名称】《中华儿科杂志》 【年(卷),期】2012(050)002
【摘要】Objective To investigate the effects of Matrigel on expression of focal adhesion kinase and on proliferation and apoptosis of alveolar epithelial cell Ⅱ of premature rat exposed to hyperoxia.Methods The primary premature rat AEC Ⅱ (gestation 19 d) were cultured in vitro. For establishing hyperoxia-exposed cell model,purified AEC Ⅱ were cultured for 12 hours after culture flasks were filled with 95％ oxygen-5％ CO2 at 5 L/min,and then sealed for 12 hours.DNA content,phosphor and total protein of FAK were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting respectively after 12 hours of air or hyperoxia exposure in the presence or absence of Matrigel.To investigate the relationship between FAK activated and proliferation or apoptosis of type Ⅱalveolar epithelial cells, levels of proliferation and apoptosis of AEC Ⅱ were measured by immunohistochemical assay of proliferating cell nuclear antigen (PCNA) and TUNEL method respectively.Results FAK and FAK-Tyr397 activity of AEC Ⅱ on Matrigel-coated substrate increased: compared with air group,the expression of PCNA decreased and apoptotic index increased
Matrigel胶对高氧损伤早产鼠肺泡Ⅱ型细胞黏着斑激酶表达变化及其对
