AIF与Calpain-Ⅰ在胃癌组织中的表达及其临床意义
赵悦;彭杰;李兴德;朱中成;张明云
【期刊名称】《现代肿瘤医学》 【年(卷),期】2013(021)003
【摘要】目的:检测胃癌组织中抑癌基因AIF与Calpain-Ⅰ mRNA水平的表达情况,探讨其在胃癌发生发展中的作用及其临床意义.方法:实时荧光定量PCR法检测64例胃癌组织及其对应的癌旁组织中AIF与Calpain-Ⅰ mRNA表达情况.结果:胃癌组织中AIF与Calpain-Ⅰ mRNA表达水平较癌旁组织上调,其阳性率分别为84％和75％,差异具有统计学意义(P＜0.05).早期胃癌中AIF与Calpain-Ⅰ mRNA表达水平增高,随着肿瘤恶性程度的增高、临床分期的变晚,逐渐表现出下调趋势.结论:在胃癌组织中,AIF与Calpain-Ⅰ mR-NA表达水平较癌旁正常组织上调,且与胃癌的临床分期显著相关.%Objective; To detect the expression level of AIF and Calpain - I mRNA in gastric cancer and discuss the correlation with occurrence and development of gastric cancer and clinical significance. Methods: Real - time fluorescence quantitative RT - PCR was used to detect AIF and Calpain -I mRNA expression level in 64 cases of gastric cancer and para - cancer normal tissues. Results; The expression level of AIF(84% ) and Calpain -I(75% ) mRNA in gastric cancer was up -regulated obviously compared to para -cancer normal tissues(P <0.05). Furthermore,the expression level of AIF and Calpain - I mRNA was upper expressed in advanced gastric cancer compared to early gastric cancer(P<0.05). With the higher degree of
AIF与Calpain-Ⅰ在胃癌组织中的表达及其临床意义
