HPLC测定多司马酯的有关物质
张广洲;马玉贞;赵仁永;张岱州
【期刊名称】《食品与药品》 【年(卷),期】2012(014)007
【摘要】目的 建立测定多司马酯中有关物质的HPLC方法.方法 采用ODS C18柱；流动相为缓冲液(冰醋酸0.4 mL,三乙胺1.6 mL,加水400 mL,用磷酸调pH至7.5)-甲醇(72∶28)；检测波长330nm.结果 样品在本色谱条件下,有关物质均能较好分离,在测定范围内有较好的线性关系及精密度.结论 测定方法简便、准确可靠,可用于多司马酯有关物质的测定.%Heparosan,a member of the glycosaminoglycan family,serves as the key precursor in heparin and heparan sulfate (HS)biosynthesis as well as their chemoenzymatic synthesis.Heparin and HS are a class of the most important glycosaminoglycans that widely used as anticoagulant drugs.The commercial heparin/HS have so far been extracted from animal source.Using heparosan as starting material followed by chemical or biological modifications to produce heparin/HS is a promising alternative way.Heparosan,which has been found as the capsular polysaccharide of certain bacteria,can be produced by extracting from microorganisms or by using recombinant enzymes in vitro.In this paper we present an overview of the production of heparosan for the preparation of bioengineered heparin and HS. 【总页数】2页(282-283)
HPLC测定多司马酯的有关物质
