349例乳腺癌HER-2基因扩增与蛋白表达相关性研究
张元;吴宁;何小燕;阮思蓓;罗霞;唐明希
【期刊名称】《中国医药科学》 【年(卷),期】2013(000)020
【摘要】目的：了解乳腺癌组织中人表皮生长因子受体2（HER-2）基因扩增与Her-2/neu蛋白表达的一致性与相关性。方法采用荧光原位杂交（FISH）法及免疫组化（IHC）法分别检测349例浸润性乳腺癌患者的HER-2基因扩增与蛋白表达情况，分析二者检测结果的一致性与相关性。结果FISH与IHC符合率为55.3%，结果存在一致性（Kappa=0.189，P=0.000），呈正相关（r=0.434，P=0.000）。结论 FISH与IHC两种检测结果存在一致性，但IHC （+～+++）患者均应以FISH检测作为评价HER-2基因是否扩增的标准方法。%Objective To investigate the correlation between human epidermal growth factor receptor 2(HER-2) gene amplification and Her-2/neu protein expression in invasive breast cancer patients. Methods Using
fluorescence
in
situ
hybridization
(FISH)
and
immunohistochemistry(IHC) method, the HER-2 gene amplification and Her-2/neu protein expression status were detected, respectively, and its concordance and correlation in a total of 164 samples of invasive breast cancer tissues. Results The overall coincidence rate of FISH and IHC was 55.3% (Kappa=0.189, P=0.000), with positive correlation (r=0.434, P=0.000) between them. Conclusion There is a concordance between the Her-2/neu protein expression and its gene amplification. HER-2
349例乳腺癌HER-2基因扩增与蛋白表达相关性研究
