Hot-nanoparticle-mediated fusion of selected cells
Azra Bahadori;Lene B.Oddershede;Poul M.Bendix
【期刊名称】《纳米研究（英文版）》 【年(卷),期】2017(010)006
【摘要】Complete fusion of two selected cells allows for the creation of novel hybrid cells with inherited genetic properties from both original cells.Alternatively,via fusion of a selected cell with a selected vesicle,chemicals or genes can be directly delivered into the cell of interest,to control cellular reactions or gene expression.Here,we demonstrate how to perform an optically controlled fusion of two selected cells or of one cell and one vesicle.Fusion is mediated by laser irradiating plasmonic gold nanoparticles optically trapped between two cells (or a vesicle and a cell) of interest.This hot-particle-mediated fusion causes total mixing of the two cytoplasms and the two cell membranes resulting in formation of a new hybrid cell with an intact cell membrane and enzymatic activity following fusion.Similarly,fusion between a vesicle and a cell results in delivery of the vesicle cargo to the cytoplasm,and after fusion,the cell shows signs of viability.The method is an implementation of targeted drug delivery at the single-cell level and has a great potential for cellular control and design. 【总页数】12页(2034-2045) 【关键词】
【作者】Azra Bahadori;Lene B.Oddershede;Poul M.Bendix
【作者单位】Blegdamsvej 17, Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark;Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, 2100 Copenhagen, Denmark;Blegdamsvej 17, Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark;Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, 2100 Copenhagen, Denmark;Blegdamsvej 17, Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark 【正文语种】英文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_nano-research-english_thesis/0201243341104.html 【相关文献】
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Hot-nanoparticle-mediated fusion of selected cells
