β淀粉样蛋白诱导小胶质细胞的炎性上清对神经元凋亡的影
响
李玮;索爱琴;张杰文;赵建华;葛继晖;秦得营;贺显军;姜晓峰
【期刊名称】《中华医学杂志》 【年(卷),期】2011(091)003
【摘要】目的 研究β淀粉样蛋白诱导小胶质细胞的炎性上清对神经元凋亡的影响.方法 用浓度为125 hmol/L的Aβ1-42诱导的小胶质细胞炎性上清干预神经元,检测Bcl-2、Caspase-3、PARP的表达及神经元凋亡的情况.结果 炎性上清液干预组Caspase-3(14.2±1.8)、Bcl-2阳性细胞数(10.6±0.8)明显高于Aβ1-42干预组(2.2±0.6,5.0±0.3)(P＜0.01),吖啶橙免疫荧光结果显示炎性上清液处理组与Aβ1-42直接干预组48 h和72 h凋亡率比较差异有统计学意义(P＜0.05).结论 Aβ1-42诱导小胶质细胞的炎性上清可以通过Caspase-3途径诱导神经元凋亡,小胶质细胞活化引起的慢性炎性过程是Aβ引起神经细胞凋亡的重要条件之一.%Objective To study the effects of neuronal apoptosis under the induction of β-amyloid inflammatory supernatant. Methods The neurons
were
intervened
by
β-amyloid-induced
inflammatory
supernatant at the concentration of Aβ1-42 at 125 nmol/L. And the expressions of Bcl-2, caspase-3, PARP and neuronal apoptosis were detected. Results The caspase-3 ( 14. 2 ± 1.8 ), Bcl-2 ( 10. 6 ± 0. 8 ) positive cells of microglia inflammatory supernatant stimulated group were significantly elevated than the control(2. 2 ± 0. 6,5. 0 ± 0. 3, P ＜ 0. 01 ). Nuclear chromatin was uniform yellow-green fluorescent. And
β淀粉样蛋白诱导小胶质细胞的炎性上清对神经元凋亡的影响
