钙蛋白酶抑制剂对缺血-再灌注心肌肌浆网钙泵功能及其蛋
白水平的影响
王金桂;祝宝华;于晓頔;陈开祥;奚群英;刘晨
【期刊名称】《江苏医药》 【年(卷),期】2011(037)002
【摘要】目的 研究钙蛋白酶抑制剂ALLN对缺血-再灌注(I-R)大鼠心肌肌浆网钙泵(SERCA)功能的影响.方法 24只雄性SD大鼠随机分为正常灌注组(对照组)、I-R组、ALLN+I-R组和二甲亚砜+I-R组(DMSO+I-R组),分别检测再灌注15 min时冠状动脉流出量(CF)和冠脉流出液中乳酸脱氢酶(LDH)漏出量;荧光分光光度计测定荧光强度来反映钙蛋白酶活性;分别采用改良p-NPP法和Millipore过滤技术测定SERCA的活性和ATP依赖的SERCA<'45>Ca2+摄取率,免疫印迹法检测心肌细胞SERCA蛋白表达水平.结果 与I-R组相比,ALLN+I-R组CF、SERCA蛋白表达水平、SERCA的活性和<'45>Ca2+摄取率显著增高(P＜0.01),而LDH漏出量和钙蛋白酶活性显著降低(P＜0.01).结论 ALLN对离体大鼠心脏I-R损伤有保护作用,与其抑制钙蛋白酶活性,一定程度减轻FR损伤对SERCA功能及蛋白表达水平的影响有关.%Objective To investigate the protective effects of calpain inhibitor(ALLN) on sarcoplastic reticulum Ca2+-ATPase (SERCA) in isolated ischemia-reperfusion (I-R) hearts of rat.Methods Twenty-four male SD rats were randomly divided into 4 groups of control, I-R, ALLN+I-R,and DMSO+I-R with 6 rats each. At 15 min after reperfusion,coronary flux(CF) and LDH were examined,the activity of calpain was detected by fluorospectrophotometer, and SERCA activity
and 45Ca2+ uptake were evaluated with modified p-NPP and Millipore filting. The protein expression of SERCA was detected by immunoblotting. Results Compared with group I-R, CF, protein expression level and activity of SERCA and 45Ca2+ uptake in group ALLN＋I-R were all significantly increased (P＜0.01), but LDH and the activity of calpain were significantly decressed (P＜0. 01). Conclusion ALLN has a significant protective effect on I-R injury of isolated hearts, which could be associated with the depression of calpain activity and reduction of the effect of I-R injury on the protein expression of SERCA. 【总页数】4页(138-141)
【关键词】缺血-再灌注损伤;钙蛋白酶;肌浆网钙泵 【作者】王金桂;祝宝华;于晓頔;陈开祥;奚群英;刘晨
【作者单位】214037,无锡市中医医院ICU;扬州市,东南大学附属扬州医院心血管病研究所;扬州市,东南大学附属扬州医院心血管病研究所;扬州市,东南大学附属扬州医院心血管病研究所;扬州市,东南大学附属扬州医院心血管病研究所;扬州市,东南大学附属扬州医院心血管病研究所 【正文语种】中文 【中图分类】R541 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_jiangsu-medical-journal_thesis/0201249101969.html 【相关文献】
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钙蛋白酶抑制剂对缺血-再灌注心肌肌浆网钙泵功能及其蛋白水平的影响
