中国民族医药杂志2019年8月第25卷第8期41药物研究HPLC法同时测定蒙药给喜古讷-6中芦荟大黄素、大黄酸、
大黄素、大黄酚、大黄素甲醞的含量△文花〉刘燕$董武3白玉霞**
(1.内蒙古民族大学蒙医药学院2017级硕士研究生，内蒙古通辽028000；2.内蒙古民族大学蒙医药学院2018级硕士研究生，内蒙古 通辽028000;3.内蒙古民族大学动物科技学院，内蒙古通辽028000)摘要：目的：建空HPLC法同时测定给喜古讷-6中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲瞇的含量。方法： 采用高效液相色谱法Welchrom C*色谱柱(4.6mmx300mm,5p.m),以甲醇-0.1 %磷酸水溶液(68：32)为流动相，流速ImL■
min-',检测波长254nm,柱温40七。结果：5种成分在各自范围内均呈良好的线性关系(rMO.9991 );平均加样回收率
95.73%~102.91%,RSD 1.3%~1.6%。结论：高效液相色谱法灵敏度高，该实验方法简便快捷、重现性好，为完善蒙药给喜
古讷-6汤的质量控制提供依据。关键词：给喜古讷-6;芦荟大黄素；大黄酸；大黄素；大黄酚；大黄素甲瞇；高效液相色谱法中图分类号:R291.2 文献标识码:A 文章编号:1006-6810(2019)08-0041-03HPLC simultaneous determination of aloe -emodin, rhein, emodin, chrysophanol and physcion in Mongolian medicine
Geixigune-6WEN Hua1, LIU Yan2, DONG Wu3, BAI Yu-xia(1. Master degree candidate of 2017 from Mongolian Medicine and Pharmacy College of Inner Mongolia University for Nationalities, 028000; 2. Master degree candidate of 2018 from Mongolian Medicine and Pharmacy College of Inner Mongolia
University for Nationalities, 028000; 3. College of Animal Science and Technology, Inner Mongolia University for Nationalities,
028000)[Abstract | Objective: HPLC method was established for simultaneous determination of Geixigune -6, Aloeemodin, Rodin, Emodin, Chrysophanol, Physcion. Method: High performance liquid chromatography was performed by Welchrom Ci8 column (4.6mmx300mm, 5|xm )The aqueous solution of methanol 0.1% phosphoric acid (68：32) was used as the mobile phase, The flow rate was ImL ? min _I.The detction wavelength was 254nm,and the column temperature was 30T . Result:The five
components showed good linear relationship in their respective range (rM0.9991). The average recovery was 95.73% -
102.91% RSD 1.3%~ 1.6.Conclusion : High performance liquid chromatography has high sensitivity, this experimental method is simple and fast, with good reproducibility, accurate and reliable results, providing a scientific basis for improving the quality control standard of Mongolian medicine for Geixigune-6 decoction.[Key words ] Geixigune-6; Aloeemodin; Rhodin; Emodin; Chrysophanol; Physcion; HPLC蒙药给喜古讷-6汤记载于《内蒙古蒙成药标准》，蒙药 疼痛等病症。大黄为方中君药,因此用高效液相色谱法对方
给喜古讷亠汤由大黄、沙棘、山奈、芒硝、木香、碱花六味药组 成，具有活血、化瘀、调经功能冋。主要用于血瘀、闭经、小腹
中大黄所含大黄酸、大黄素、芦荟大黄素进行了含量测定研 究,为完善蒙药给喜古讷-6的质量控制提供科学依据1仪器与试药△基金项目：内蒙古民族大学特色交叉学群科建设项目 （MDXK008）日本岛津高效液相色谱仪;SQP型1/10万电子分析天 平【赛多利斯科学仪器（北京）有限公司］,KQ5200DB型超声
作者简介:文花，女，在读硕士研究生,从事蒙药材及蒙成药质量标 准研究。E-mail:m 15047530084@ 163.como波清洗器（江苏省昆山市淀山湖镇）。大黄酸对照品（批号:DR191229,纯度：M98%）、大黄
*通讯作者:白玉霞，女，博士，教授，硕导，从事蒙药材及蒙成药质 量标准研究。E-mail: byx6088@ 163 .com 0
素对照品（批号:P322005，纯度：M 98%）、大黄酚对照品（批
HPLC法同时测定蒙药给喜古讷-6中芦荟大黄素、大黄酸、大黄素、大黄 - 论文
