Increased Expression of the NOD-like Receptor Family, Pyrin Domain Containing 3 Inflammaso

Increased Expression of the NOD-like Receptor Family, Pyrin Domain Containing 3 Inflammasome in Dermatomyositis and Polymyositis is a Potential

Contributor to Their Pathogenesis

Xi Yin;Gen-Cheng Han;Xing-Wei Jiang;Qiang Shi;Chuan-Qiang Pu

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2016(129)009

【摘要】Background:Dermatomyositis (DM) and polymyositis (PM) are common

inflammatory

myopathies

whose

immunopathogenic

mechanisms remain poorly understood.The NOD-like receptor family,pyrin domain containing 3 (NLRP3) inflammasome is a type of cytoplasmic multiprotein inflammasome and is responsible for the activation of inflammatory reactivations.Responding to a wide range of exogenous and endogenous microbial or sterile stimuli,NLRP3 inflammasomes can cleave pro-caspase-1 into active caspase-1,which processes the pro-inflammatory cytokines pro-interleukin (IL)-1 β and pro-IL-18 into active and secreted IL-1 β and IL-18.The NLRP3 inflammasome is implicated in infectious and sterile inflammatory diseases.However,it remains unclear whether it is involved in the pathogenesis of DM/PM,which we aim to address in our research.Methods:In this study,22 DM/PM patients and 24 controls were recruited.The protein and RNA expression of IL-1β,IL-18,NLRP3,and

caspase-1 in serum and muscle samples were tested and compared between the two groups.Results:The serum IL-1β and IL-18 levels were significantly higher in DM/PM patients than those in the controls by enzyme linked immunosorbent assay (ELISA,DM vs.control,25.02 ± 8.29 ng/ml vs.16.49 ± 3.30 ng/ml,P ＜ 0.001; PM vs.control,26.49 ± 7.79 ng/ml vs.16.49 ± 3.30 ng/ml,P ＜ 0.001).Moreover,the real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) showed that DM/PM patients exhibited higher RNA expression of IL-1 β,IL-18,and NLRP3 in the muscle (for IL-1β,DM vs.control,P =0.0012,PM vs.control,P =0.0021; for IL-18,DM vs.control,P =0.0045,PM vs.control,P =0.0031;

for

NLRP3,DM

vs.control,P

=0.0017,PM

vs.control,P

=0.0006).Moreover,the protein expression of NLRP3 and caspase-1 in muscle samples of DM/PM patients were also significantly elevated compared to that in the muscles of the controls.Conclusions:Our findings demonstrate that the NLRP3 inflammasome is implicated in the pathogenesis of DM/PM.High NLRP3 expression led to elevated levels of IL-1β and IL-18 and could be one of the factors promoting disease progress.

【总页数】6页(1047-1052) 【关键词】

【作者】Xi Yin;Gen-Cheng Han;Xing-Wei Jiang;Qiang Shi;Chuan-Qiang Pu

【作者单位】Department of Neurology, Chinese People's Liberation Army General Hospital, Beijing 100853, China;Department of Immunology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China;Department of Immunology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China;Department of Neurology, Chinese People's

Liberation

Army

General

Hospital,

Beijing

100853,

China;Department of Neurology, Chinese People's Liberation Army General Hospital, Beijing 100853, China 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-medical-journal_thesis/0201254255611.html 【相关文献】

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