黄槿内生真菌的次级代谢产物及其生物活性研究
李丹;朱天骄;顾谦群;李德海
【期刊名称】《中国海洋药物》 【年(卷),期】2012(031)006
【摘要】目的 对1株分离自广西红树林植物黄槿(Hibiscus tiliaceus)的内生真菌Penicillium sp.LD-68的抗肿瘤活性成分进行研究.方法 采用溶剂萃取、柱色谱层析及制备HPLC等方法对菌株发酵产物进行活性追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以SRB和MTT法评价化合物的抗肿瘤活性.结果 从中分离得到5个弯孢霉菌素类化合物,其结构分别鉴定为curvularin(1)、dehydrocurvularin(2)、11-β-Hydroxy-12-Oxocurvularin(3)、11-β-Hydroxycurvularin(4)、11-α-Hydroxycurvularin(5).化合物1～5对A549、hela、BEL-7402、K562 4种人肿瘤细胞株具有不同程度的抑制作用,其中化合物2、3、4、5对hela细胞具有较好的抗肿瘤细胞活性,IC50分别为3.99、7.75、10.00、5.10 μmol·L-1.结论 首次测定了化合物3～5对A549、hela、BEL-7402、K562 4种肿瘤细胞系的细胞毒活性,其中对hela细胞显示出较高的抑制率.%Objective To investigate the antitumor bioactive ingredient of a endophytic fungus Penicillium sp. LD-68 isolated from Guang Xi mangrove plant Hibiscus tiliaceus L.. Methods The extract of the fungus was purified by solvent extraction, column chromatography and preparative HPLC guided by the bioassay results. Their structures were established by physicochemical properties and spectroscopic a-nalysis. The antitumor activities were evaluated by SRB method and MTT
method. Results Five compounds were identified as curvularin (1), dehydrocurvularin
(2),
ll-β-Hydroxy-12-Oxocurvularin
(3),
ll-β-
Hydroxycurvularin (4), 11-α-Hydroxycurvularin (5). Compounds 1-5 showed inhibitory activities to four kinds of human tumor cell lines including A549, hela, BEL-7402 and K562, and compounds 2～5 have more potential antitumor activities against hela cells, with IC50 values of 3. 99, 7. 75, 10. 00 and 5.10μM, respectively. Conclusion Compounds 3～5 are reported their cytoxic activities against the four kinds of human tumor cell lines, A549, hela, BEL-7402 and K562 for the first time, and exhibitingmore selectivities to hela cell line. 【总页数】6页(17-22)
【关键词】红树林内生真菌;Hibiscus tiliaceus;Penicillium sp.;弯孢霉菌素;抗肿瘤
【作者】李丹;朱天骄;顾谦群;李德海
【作者单位】教育部海洋药物重点实验室,中国海洋大学医药学院,山东青岛,266003;教育部海洋药物重点实验室,中国海洋大学医药学院,山东青岛,266003;教育部海洋药物重点实验室,中国海洋大学医药学院,山东青岛,266003;教育部海洋药物重点实验室,中国海洋大学医药学院,山东青岛,266003 【正文语种】中文 【中图分类】R931 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-marine-drugs_thesis/0201241594089.html 【相关文献】
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黄槿内生真菌的次级代谢产物及其生物活性研究
