严重多发伤患者外周血清对巨噬细胞核因子-κB的激活作
用及其与预后的关系
梁华平;徐祥;夏浩;费军;吴丹;王正国
【期刊名称】《中华外科杂志》 【年(卷),期】2009(047)001
【摘要】目的 探讨严重多发伤患者伤后24 h外周血清对巨噬细胞核因子-κB(NF-κB)的激活作用及其与预后的关系.方法 收集47例严重多发伤患者及24名健康体检者的外周血清.将带有荧光素酶报告基凶的NF-κB重组质粒转染至巨噬细胞(RAW 264.7),24 h后用不同的血清刺激该细胞6 h.榆测荧光素酶活性以反映血清对NF-κB的激活作用强度,同时以EIJSA法测定血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白细胞介素-10(IL-10)、白细胞介素-1(IL-1)受体拮抗剂(IL-1Ra)、可溶性肿瘤坏死因子受体Ⅰ(sTNFRI)水平.结果 创伤血清组对巨噬细胞NF-κB的激活作用显著高于正常血清组,且创伤后MODS组、死亡组值分别显著高于尤MODS组及存活组.该变化与APACHE Ⅱ评分呈明显的正相关,但与血清中各细胞因子水平无明显相关性.NF-κB活性在判定MODS及死亡结局的受试者作业特征曲线(ROC)下面积显著大于APACHE Ⅱ评分的ROC下面积.结论 早期测定严重多发伤患者外周血清对巨噬细胞NF-κB的激活作用对MODS及死亡结局的判定具有参考价值.%Objective To investigate the stimulating effect of sera from severe multiple trauma patients within 24 h pest trauma on nuclear factor-κB(NF-κB) activity in macrophage and their relationship with patients prognosis. Methods Peripheral blood of 47 patients with multiple traumas with injury
severity score(ISS) ≥ 16 and 24 healthy volunteers were obtained, and sera samples were isolated. And 24 h after transfection of the recombinant NF-κB plasmid containing luciferase reporter gene into the mouse macrophage line ( RAW 264. 7), the cells were stimulated by sera from different patients for 6 h, then stimulating effect of sera on NF-κB was assessed by luciferase activity. The concentrations of interleukin-1β, tumor necrosis factor α, interleukin-10, interleukin-1 receptor antagonist, and soluble tumor necrosis factor receptor Ⅰ were detected with ELISA kits. Results The stimulating activity of sera from trauma patients on NF-κB was increased significantly, and it was higher in MODS group, nonsurvivor group than that in non-MODS, survivor group respectively. The level of activity was correlated pesitively with APACHE Ⅱ score, while it did not have relationship to the cytokine or endogenous antagonist levels. The area under the receiver operating characteristic curve(ROC) of NF-κB activity for predicting MODS and mortality was significantly higher than that of APACHE Ⅱ score. Conclusion Early measurement of NF-κB stimulating activity of sera from severe multiple trauma patients may have the value to predict occurrence of MODS and mortality. 【总页数】4页(54-57)
【关键词】多处创伤;NF-κB;巨噬细胞;预后 【作者】梁华平;徐祥;夏浩;费军;吴丹;王正国
【作者单位】400042重庆,第三军医大学大坪医院野战外科研究所一室,创伤、烧伤与复合伤国家重点实验室;400042重庆,第三军医大学大坪医院野战外科研究所一室,创伤、烧伤与复合伤国家重点实验室;400042重庆,第三军医大学大坪医院野战外科研究所一室,创伤、烧伤与复合伤国家重点实验室;400042重庆,第三军医大学大坪医院野战外科研究所一室,创伤、烧伤与复合伤国家重点实验室;400042重庆,第三军医大学大坪医院野战外科研究所一室,创伤、烧伤与复合伤国家重点实验室;400042重庆,第三军医大学大坪医院野战外科研究所一室,创伤、烧伤与复合伤国家重点实验室 【正文语种】中文 【中图分类】R6 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-surgery_thesis/0201234900306.html 【相关文献】
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严重多发伤患者外周血清对巨噬细胞核因子-κB的激活作用及其与预后的关系
