烧伤血清作用下心肌细胞蛋白激酶B和p38丝裂原活化蛋
白激酶通路的交叉对话研究
吕根法;陈璧;张万福;王耘川;朱雄翔;胡大海
【期刊名称】《中华烧伤杂志》 【年(卷),期】2008(024)004
【摘要】Objective To investigate the possibility of erosstalk between phosphatidylinositol 3-kinase (PI 3-K)/Akt pathway and p38 mitogen-aetivated protein kinase (p38MAPK) pathway in cardiomyocyte with challenge of burn serum, and to explore their influence on eardiomyocyte injury after bum. Methods The model of murine cardiomyocyte with stimulation of burn serum was established. ( 1 ) The level of Akt and p38 phosphorlation in cardiomyocyte were examined with stimulation of 10% bum serum before stimulation and 1,3,6,12,24 hour after stimulation. (2) The levels of Akt and p38 phosphorylation in cardiomyocyte were determined with stimulation of burn serum (at concentration of 5% ,10% ,20% ) or 10% burn serum plus insulin( at concentration of 1 × 10-8, 1 × 10-7,1 × 10-6mol/L). The content of creatine kinase (CK) in superuate was also detected. (3)Addition to the inhibitor of p38 MAPK pathway(SB203580) and PI3K/ Akt pathway ( LY294002 ), the level of p38 M APK , PI3 K/Akt and the content of CK in supernate were deter- mined. Results (1)The level of p-p38 in cardiomyocyte was 4.0±0.8,3.6±0.8,5.1±1.6,2.4± 0.5,3.0 ± 0.6 at
烧伤血清作用下心肌细胞蛋白激酶B和p38丝裂原活化蛋白激酶通路的
