食管癌高发区HPV检测及与p53的关系

食管癌高发区HPV检测及与p53的关系

陆哲明;郭梅;李涛;柯杨;陈克能;邢海平;梅强;杨怀华

【期刊名称】《中华肿瘤杂志》 【年(卷),期】2001(023)003

【摘要】Objective To investigate the association of HPV with the development of esophageal cancer(EC) in a high-incidence area of EC and to elucidate its correlation with p53 overexpression.Methods Thirty EC specimeas were collected from Anyang, Henan. Four pairs of primers were designed to perform in situ hybridization (ISH）and in situ PCR（ISPCR）. Immunohistochemical staining was used to detect p53.Results HPV L1, HPV-16-E6 and HPV-16-E7 was detected in 10.0%,60.0% and 63.3% of the EC samples, respectively. The detection rate of HPV-18-E6 was low(6.7%) and no EBV was detected. Overexpression of p53 was identified in 73.3% EC. With ISH or ISPCR, HPV-16-E6 was positive in 53.3% of EC. Conclusion The low detection rate of HPV L1 and high detection rate of HPV-16-E6 and E7 genes suggest that HPV may be partially lost when inte-grating into tumor cell genome，while E6 and E7 genes are intact. The results support a role of HPV-16 in the pathogenesis of EC in high incidence area. Although p53 mutation takes an important part in tumor pathogenesis, it is not consistent with the HPV existence in the EC cells.%目的探讨食管癌高发区中人乳头瘤病毒（HPV）、爱泼斯坦-巴尔病毒（EBV）与食管癌发病的关系，