HPLC同时测定不同种葛根中3种异黄酮成分的含量
楚纪明;马树运;李海峰;张琳;徐立
【期刊名称】《食品与药品》 【年(卷),期】2015(000)001
【摘要】目的：建立HPLC同时测定野葛、粉葛、泰国葛中异黄酮葛根素、大豆苷和染料木素含量的方法。方法色谱柱为Hypersil BDS C18柱（250 mm×4.6 mm，5μm）；流动相为甲醇-水，梯度洗脱，流速0.7 mL/min，进样量10μL，柱温25℃，检测波长250 nm。结果葛根素、大豆苷和染料木素分别在0.05~1.20μg，0.025~0.60μg，0.025~0.60μg范围内呈良好线性关系，r分别为0.9999，0.9999，0.9999。其平均回收率为97.26%~104.64%，RSD均小于3%。结论建立的方法简单、快捷，重复性好，结果准确可靠，可为葛根的资源研究提供支持。%Objective To establish a HPLC method for simultaneous determination of puerarin, daidzin, and genistein from Pueraria lobata (wild) Ohwi, Pueraria thomsonii Benth and Pueraria mirifica. Methods The Hypersil BDS C18 column (250 mm×4.6 mm, 5μm) was adopted. The mixture of methanol and water was used as mobile phase of gradient elution, with the flow rate of 0.7 mL/min. The injection volume was 10μL. The Column temperature was 25℃, and the detection wavelength was 250 nm. Results This method had good linearity in the range of 0.05-1.20μg, 0.025-0.60μg and 0.025-0.60μg for puerarin, daidzin and genistein, respectively, with r of 0.9999, 0.9999 and 0.9999, respectively. The average recoveries were 97.26%-104.64%,
HPLC同时测定不同种葛根中3种异黄酮成分的含量
