葡萄糖调节蛋白78在子痫前期胎盘滋养细胞中的表达及意
义
王耸;瓮占平;纪向虹
【期刊名称】《实用妇产科杂志》 【年(卷),期】2012(028)001
【摘要】Objective: To investigate the relevance of the expression of glucose regulated protein 78 (GRP78) in placenta with the pathogenesis of preeclampsia(PE). Methods:A total of 120 pregnant women with PE who were hospitalized in Qingdao Municipal Hospital and The eighth People's Hospital of Qingdao from Jan. 2009 to Dec. 2009 were recruited in this study and divided into four groups: early mild PE group (n =30), late mild PE group(n =30), early severe PE group (? =30), late severe PE group (re =30). 30 normal pregnancy were chosen as control. The expression of GRP78 mRNA and protein in placenta were measured by RT-PCR, immunohistochemistry and Western Blotting. Results:?The expressions of GRP78 mRNA in different PE groups were significantly higher than that in control group (P<0. 05). However, there were no significant differences of GRP78 mRNA levels among different PE groups ( P > 0. 05). ?GRP78 protein expressed in the cytoplasma of trophoblasts of all the PE groups and normal control, manifested as the clear brown particles. Western blotting revealed that the expression level of GRP78 in placenta of all the PE groups were markedly higher
葡萄糖调节蛋白78在子痫前期胎盘滋养细胞中的表达及意义
