Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination

Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with

Streptococcus iniae

ZHAN Wenbin;LIU Hongming;XING Jing;SHENG Xiuzhen;TANG Xiaoqian

【期刊名称】《中国海洋湖沼学报（英文版）》 【年(卷),期】2009(027)003

【摘要】A pathogenic bacterium (S636), identified as Streptococcus iniae, was isolated from turbot (Scophthalmus maximus) in 2005. We immunized turbot with formalin-killed S. iniae four times (on days 1, 14, 21, and 28) by intraperitoneal inoculation. After each vaccination, we obtained serum samples and isolated the lymphocytes from the peripheral blood, spleen, pronephros, and mesonephros. We measured surface Ig-positive (sIg+) lymphocytes and serum antibody levels from these organs using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively, using monoclonal antibodies against turbot immunoglobulin. We confirmed that the antibody reacted with both the surface and plasma Ig by confocal laser scanning microscopy and electron microscopy. The percentage of sIg+ in the lymphocytes increased following each successive vaccination. The mean percentage increased from 31.96% (control) to 37.49%, 38.36%, 42.9%, and 51.63% in the peripheral blood; from 27.09% to 36.63%, 36.81%, 39.28%, and 46.0% in the spleen; from 22.2% to 28.99%, 29.21%, 32.83%, and 41.58%