Phytochemical analysis, in vitro antioxidant and antibacterial activities of root extracts

Phytochemical analysis, in vitro antioxidant and antibacterial activities of root extracts of Carduus

macracanthus

Mathewos Anza[1];Mesfin Bibiso[1];Bereket Alemayehu[2];Engeda Desalegn[3];

【期刊名称】《海岸生命医学杂志(英文版)》 【年(卷),期】2017(005)011

【摘要】Objective: To investigate phytochemical constituents, antibacterial and antioxidant activity of the root extracts of Carduus macracanthus (C. macracanthus). Methods: Phytochemical screening tests were conducted as per of the standard procedure to identify the classes of compounds present in the root extract of C. macracanthus. Silica gel column chromatographic technique was applied to separate the constituents from chloroform/methanol (1:1) root extracts. Various spectroscopic techniques were applied to determine the structure of the isolated compound. In vtiro antibacterial activities of crude extracts were evaluated via disc diffusion method. Minimum inhibitory concentration and minimum bacterial concentration were determined by broth dilution method. Moreover, antioxidant activities of extracts were determined by DPPH assay, the phosphomolybdenum method and total flavonoid contents were determined as per of the standard procedures. Results: Phytochemical screening of root extracts of C. macracanthus revealed the presence alkaloids, flavonoids, triterpenes,