法莫替丁与扑尔敏联合应用治疗乙酸致胃溃疡的协同作用
陈超;覃珍
【期刊名称】《实用医学进修杂志》 【年(卷),期】2005(033)004
【摘要】Objective: Previous work demonstrates that H2 receptors antagonist shows gastroprotective effect in the ethanol, aspirin and pilorous ligature-induced gastric ulcer in rats as well as in the ethanol/hydrochloric acid-induced ulcer in rats ,and H1-receptor antagonists have been reported to be potent anti-inflammatory compounds. The aim of the present study was designed to assess the synergistic action of famotidine and chlorpheniramine in the acetic acid-induced chronic gastric ulcer model in rats. Methods:Chronic gastric lesions were induced in male Sprague-Dawley rats with serosal application of acetic acid. 40 SD rats were randomly divided into 5 groups:blank
group,control
group,
famotidine
(FMD)
group,
chlorpheniramine (CPA) group and FMD+CPA group; Every group was given intraperitoneally(i.p.) distilled water 0.5 ml/100 g、the same volume of 0.9% saline、FMD4 mg/kg、CPA10mg/kg、FMD+CPA (the same dose) respectivedly daily for 10 days. On days 10,the ulcer area was determined by planimetry, The levels of MPO in the liver homogenation was measured by bio-chemical methods and the plasma levels
of 6-keto-PGF1a and IL-8 by radio-immune assay
methods.Results:although FMD or CPA alone possess a potent antiulcer or anti-inflammatory activity. The synergistic effects of FMD+CPA were confirmed in the lesion area, IL-8,6-keto-PGF1a and MPO. The effect of FMD+CPA was significantly different as compared to the control and FMD reducing the lesion area (mm2) from 40.18+/-2.6 in controls to 6.83+/-2.97,P<0.01; 32.9+/-3.27 in FMD to 6.83+/-2.97, P<0.01; the plasma levels of IL-8 decreased 0.69+/-0.11→0.4+/-0.04ng· L- 1 in controls,P<0.01,0.51+/-0.08 in the FMD→0.4+/-0.04ng· L-1,P<0. 05;the lever of 6-keto-PGF1a increased 7.55+/-1.65 in controls→16.62+/-0.97pg/ml,P<0.01,13.15+/-1.48 in FMD→16.62+/-0.97pg/ml,P<0.05;The levels of MPO in the liver homogenation dcreased9.12+/-2.05 in controls→4.33+/-0.95,P<0.01,8.3+/-1.29
in
FMD→4.33+/-
0.95,P<0.01.Conclusion:the synergistic action of famotidine and chlorpheniramine in the acetic acid-induced chronic gastric ulcer model in rats decreased the incidence of ulcers and also enhanced the healing of ulcers.%目的:探讨H1扑尔敏、H2法莫替丁阻滞剂联合应用治疗乙酸致胃溃疡的疗效.方法:采用乙酸致胃溃疡模型,大鼠随机分为5组:正常组(Blank)、生理盐水组(Control)、法莫替丁组(famotidin,FMD)、扑尔敏组(chlorpheniramine ,CPA)、联合用药组(FMD+CPA),于造模后次日给药10 d,处死搜集标本. 用肉眼、光镜观察胃标本,按公式法计算溃疡面积;肝组织匀浆中的MPO的含量采用生化法 ;血6-酮-前列腺素F1a、白介素-8( IL-8)采用放免分析法.结果:CPA组、FMD组与FMD+ CPA联合组的溃疡面积、6-酮-前列腺
素F1a与Control组比较有显著差异性(P<0.05),FMD组与 CPA组之间无显著差异性(P>0.05),FMD+ CPA联合组与CPA组、FMD组有显著差异性(P<0.05).但是FMD+ CPA联合组与FMD组的大鼠血浆IL-8及肝组织MPO相比要明显低IL-8,P<0.05;MPO,P<0.01,提示扑尔敏有抗炎作用. 结论:联合应用H1、H2受体阻滞剂治疗胃溃疡能取得良好的疗效,尤其抗炎作用显著差异性.其机制与减少炎症因子的产生,减少对胃黏膜的损伤,改善胃黏膜血流有关. 【总页数】9页(213-221)
【关键词】法莫替丁;扑尔敏;胃溃疡 【作者】陈超;覃珍
【作者单位】三峡大学,医学院,三峡天然药物实验室,宜昌,443002;三峡大学,医学院,三峡天然药物实验室,宜昌,443002 【正文语种】中文 【中图分类】R5 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_practical-medical-training-magazine_thesis/0201256594492.html 【相关文献】
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2.阿莫西林、克拉霉素、法莫替丁联合治疗胃溃疡 [J], 张秀芹; 彭俊红 3.替普瑞酮联合法莫替丁治疗胃溃疡临床疗效观察 [J], 李洪伟 4.替普瑞酮联合法莫替丁治疗胃溃疡临床疗效观察 [J], 李洪伟 5.法莫替丁不同晶型抗胃溃疡作用的比较 [J], 浦培英
法莫替丁与扑尔敏联合应用治疗乙酸致胃溃疡的协同作用
