泛素羧基末端水解酶L5对乳腺癌细胞增殖和凋亡的影响
丁芳;马建林;吴晓巍;刘芝华
【期刊名称】《中华肿瘤杂志》 【年(卷),期】2018(040)012
【摘要】Objective To investigate the effect of UCHL5 on proliferation and apoptosis of breast cancer cells.Methods SW527 cells were infected with lentiviral vector carrying short hairpin RNA to delete the expression of UCHL5. 3-( 4, 5-dimethylthiazol-2-yl)-5-( 3-carboxymethoxyphenyl)-2-( 4-sulfophenyl)-2H-tetrazolium ( MTS ) assay was used to examine cell proliferation, and subcutaneous transplantation experiments were performed to detect tumor growth. Cell apoptosis was detected using Annexin V/ Propidium iodide (PI) double staining. The correlation between UCHL5 expression and the expressions of proliferation and apoptosis associated genes was analyzed using TCGA breast invasive carcinoma data set. The relationship between UCHL5 expression and breast cancer patients'survival was analyzed using Kaplan-Meier Plotter online tool. Results After knockdown of UCHL5, A values of SW527 cells on day 2 and day 4 were 0.822 ±0.017 and 1.045±0.023, respectively, which were significantly lower than 0.976±0.016 and 1.284±0.025 of control cells on day 2 and day 4 ( P＜0.001). In vivo xenografted mouse model, the volume in UCHL5-suppressed group was (166.90±75.05) mm3, significantly smaller than (329.80±35.84) mm3in control group (P
泛素羧基末端水解酶L5对乳腺癌细胞增殖和凋亡的影响
