EMS诱变处理金花菜种子条件分析
胡志峰;魏臻武;唐嘉
【期刊名称】《草地学报》 【年(卷),期】2017(025)002
【摘要】为确定EMS诱变处理金花菜(Medicago polymorphaL.)种子的最适条件,获得化学诱变性状稳定的突变系和育种后代.本试验以淮扬金花菜种子为材料,EMS为诱变剂,采用5个甲基磺酸乙酯(EMS)诱变浓度(o.1％,0.3％,0.5％,0.7％和0.9％),3个处理时间(3h,6h和9 h),分别对诱变后种子发芽率、胚根长度、移栽成活率及相对越冬率进行分析.研究发现EMS诱变处理对淮扬金花菜发芽率和胚根伸长有抑制效应,且EMS诱变浓度抑制效应大于诱变时间;EMS处理对淮扬金花菜移栽成活率影响较小;低浓度EMS处理后淮扬金花菜保持了较高的越冬率,但高浓度EMS抑制金花菜的越冬.0.7％ EMS 9 h组合和0.9％ EMS 6 h组合的发芽率均接近50％,最终确定0.7％ EMS诱变处理9h和0.9％ EMS诱变处理6h的组合为金花菜种子最佳诱变条件.%To optimize the condition of EMS mutagenesis of burclover (Medicago polymorpha L.) seeds,obtain mutation lines with stable behavior,we used Huaiyang burclover seed as plant material,and treated it with Ethyl methyl sulfonate (EMS),Five ethyl methane sulfonate (EMS) concentrations (0.1％,0.3％,0.5％,0.7％ and 0.9％),and 3 processing time (3 h,6 h and 9 h) were used for mutagenesis.The germination rate and radicle length,transplanting survival rate and relative survival rate were analyzed.The results showed that EMS mutagenesis time and
EMS诱变处理金花菜种子条件分析
