Relationship between Vasodilation Effect of Anisodamine and Endothelial Target for Acetylc

Relationship between Vasodilation Effect of Anisodamine and Endothelial Target for Acetylcholine on Microvessels in Rat

鲍颖霞;李庆平;汪海

【期刊名称】《生物医学研究杂志（英文版）》 【年(卷),期】2002(016)004

【摘要】Objective To study the effects of anisodamine on microvessels and its relatwn-ship with endothelium target for acetylcholine (ETA). Methods Norepinephrine precontracted mesen-teric vascular beds in rat were used to determine changes of tension of vessels in response to anisodamineafter removal of endothelium , coincubated with L-NAME, nitric oxide synthase (NOS) inhibitor, in-domethacin , cyclo-oxygenase inhibitor and glibenclamide , ATP-dependent K+ channels (K ATP ) inhibitor.Results Anisodamine produced a vasodilation effect on mesenteric vascular beds, which was remark-ably inhibited after removal of endothelium (P＜ 0.01). The vasodilation effect of anisodamine was al-so significantly inhibited after coincubated with indomethacin (1 μmol/ L), NG-nitro-L-arginine methylester ( L- NAME, 100 μrmol / L) and glibenclamide (1 μmol/L, P＜0. 01, vs control). Conclu-sion The vasodilation effect of ani.sodamine on microvessels was mediated by ETA. This is related toprostacyclin , endothelium derived relaxing factor (EDRF) and KATP besides M, a-receptor on vascularmooth muscle cell