Three-dimensional fluorescence characteristics of dissolved organic matter produced by Pro

Three-dimensional fluorescence characteristics of

dissolved organic matter produced by

Prorocentrum donghaiense Lu

ZHAO Weihong;WANG Jiangtao;CHEN Meimei

【期刊名称】《中国海洋湖沼学报（英文版）》 【年(卷),期】2009(027)003

【摘要】Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth, stationary and decline stages into <0.45 μm filtrate, 100 kDa-0.45 μm, 10-100 kDa and 1-10 kDa retentate and <1 kDa ultrafiltrate fractions. The fluorescence properties of different molecular weights of dissolved organic matter (DOM) were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration, shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size, but showed little change in the decline stage. However, the emission wavelengths in the decline stage exhibited a blue shift. Very distinct C type and A type peaks in humic-like

fluorophores were observed. With a prolonged culture time, the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift, while the A:C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction.

【总页数】6页(564-569) 【关键词】

【作者】ZHAO Weihong;WANG Jiangtao;CHEN Meimei

【作者单位】Institute of Oceanology, Chinese Academy of Sciences;Ocean University of China;Institute of Oceanology, Chinese Academy of Sciences 【正文语种】中文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-oceanic-limnology-english-edition_thesis/0201252507272.html 【相关文献】

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