Stat3信号转导通路调控Survivin表达促进结肠癌细胞凋亡

Stat3信号转导通路调控Survivin表达促进结肠癌细胞凋

亡

余力伟;马向涛

【期刊名称】《中华实验外科杂志》 【年(卷),期】2008(025)003

【摘要】目的 探讨Stat3/Survivn信号转导通路调控结肠癌细胞凋亡的作用机制.方法 用阳离子脂质体介导Stat3反义寡核苷酸(20 mol/L)转染人结肠癌HT29细胞,噻唑蓝(MTY)法检测细胞增殖状态;流式细胞术检测细胞周期与凋亡;Western blot检测Stat3、p-Stat3、Survivin与bcl-2凋亡家族成员bcl-2、bcl-Xl于bax的表达.结果 Stat3反义寡核苷酸作用HT29细胞72 h后,G1期细胞比率由61.4%上升至75.6%,S期细胞比率由19.4%下降至8.6%,细胞增殖受抑制.凋亡细胞百分比由5.6%增加至22.1%,促进细胞凋亡.Stat3、p-Stag、Cychn D1与Survivin表达下降,bcl-2、bcl-Xl与bax变化不明显.结论 阻断Stat3通路可以抑制靶基因Survivin表达并诱导结肠癌细胞凋亡.%Objective To study the role of Stat3/Survivin pathway in the process of apoptosis in colon cancer cens.Methods Human colon cancer cell line HT29 was transfected with Stat3 antisense oligonucleotide(Stat3 AS-ON)(20 mol/L)mediated by liposome.MTT assay was used to measure the proliferation.Flow eytometry was applied to analyze the cell cycle and apoptosis.The expression of Stat3,pStat3,cyclin D1,Survivin,bcl-2,bcl-xL and bax was detected by Western blot.Results After the treatment of HT29 cells with Star3 AS-ON for 72 h,the percent of cells in G1 phase