Inhibitory effect of polyphenolic-rich extract from Cola nitida (Kolanut) seed on key enzy

Inhibitory effect of polyphenolic-rich extract from Cola nitida (Kolanut) seed on key enzyme linked to

type 2 diabetes and Fe2+ induced lipid peroxidation in rat pancreas in vitro

Ganiyu Oboh;Kate E Nwokocha;Ayodele J Akinyemi;Adedayo O Ademiluyi

【期刊名称】《亚太热带生物医学杂志（英文版）》 【年(卷),期】2014(000)0z1

【摘要】Objective: To investigate the inhibitory effect of phenolic-rich extracts from Cola nitida (C.nitida ) seeds on key enzymes linked with type-2 diabetes and Fe2+ induced oxidative stress in rat pancreas.Methods:antioxidant properties and inhibitory effect of the extract on α - amylase and α - glucosidase as well as on Fe2+ induced lipid peroxidation in rat pancreas were determined in vitro.Results:The phenolic extract was prepared with 80% acetone (v/v). Subsequently, theα-glucosidase (EC50=0.32 mg/mL) activities as well as Fe2+ induced lipid peroxidation in rat pancreas in a dose dependent manner. In addition, the extract had high DPPH radical scavenging ability (EC50=2.2

mg/mL)

and

reducing

power

(8.2

mg

AAE/g).

Characterization of the main phenolic compounds of the extract using gas chromatography analysis revealed catechin (6.6 mg/100 g), epicatechin (3.6 mg/100 g), apigenin (5.1 mg/100 g) and naringenin (3.6 mg/100 g) were the main compounds in the extract.Conclusions:This

antioxidant and enzyme inhibition could be some of the possible mechanism The result revealed that C. nitida extract inhibited α-amylase (EC50=0.34 mg/mL) and by which C. nitida is use in folklore for the management/treatment of type-2 diabetes. However, the enzyme inhibitory properties of the extract could be attributed to the presence of catechin, epicatechin, apigenin and naringenin. 【总页数】8页(405-412) 【关键词】

【作者】Ganiyu Oboh;Kate E Nwokocha;Ayodele J Akinyemi;Adedayo O Ademiluyi

【作者单位】Department of Biochemistry, Federal University of Technology, P.M.B., 704, Akure 340001, Nigeria;Department of Biochemistry, University of Ibadan, Ibadan, Nigeria;Department of Biochemistry, Federal University of Technology, P.M.B., 704, Akure 340001, Nigeria;Department of Biochemistry, Federal University of Technology, P.M.B., 704, Akure 340001, Nigeria 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_asia-pacific-tropical-biomedical-journal-english_thesis/0201254084247.html 【相关文献】

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