胃癌组织runt相关转录因子3基因表达与其甲基化状态的
关系
高楠;陈卫昌;岑建农
【期刊名称】《中华肿瘤杂志》 【年(卷),期】2008(030)005
【摘要】Objective This study was designed to clarify the significance of DNA methylation in the expression of runt-related transcription factor 3 (Runx3) gene. Methods Reverse transcription-PCR (RT-PCR) was used to measure the expression level of Runx3 mRNA in paired samples of primary gastric cancer and corresponding non-cancerous gastric mucosa, taken from surgical specimens of 70 gastric cancer patients. Western blot was used to detect the protein expression level of Runx3 gene. The promoter methylation status of Runx3 gene was detected by methylation specific PCR (MSP). Furthermore, RT-PCR was used to mesure the expression of DNA methyltransferase 1 (Dnmt1) mRNA . The correlation of Runx3 expression and methylation with Dnmt1 mRNA expression was analyzed. Results The mRNA expression level of Runx3 gene was significantly lower in gastric cancer than that in the matched normal gastric mucosa (0.5740±0.3580 vs. 1.7250± 0.4080, P<0.05), and the Runx3 protein expression level in gastric cancer was also significantly lower than that in the matched normal gastric mucosa (P<0.05). Promoter hypermethylation of Runx3 gene was detected in
胃癌组织runt相关转录因子3基因表达与其甲基化状态的关系
