多药耐药基因转染对人胎盘源性间充质干细胞生物学特性
影响研究
韩丽英;叶明珠;李荷莲;王博蔚;王强
【期刊名称】《中华医学杂志》 【年(卷),期】2009(089)039
【摘要】Objective To transfer multidrug resistance gene (mdr1) into human placental mesenchymal stem cells (P-MSCs) by retroviral vector and assess the effects of mdr1 gene transduction upon biological features of P-MSCs.Methods Human P-MSCs were isolated from trypsin-digested term placentas and then transduced by reconstructed retroviral vector containing mdr1 gene and green fluorescent protein (GFP) reporter gene.Flow cytometric analysis was employed to determine the immunophenotypes of transfected P-MSCs.And the proliferation and cell cycle were detected by methyl thiazolyl tetrazolium and propidium iodide staining.Uhrastructures of transfected P-MSCs were observed and different induction conditions used to direct the cells to differentiate into adipocytes and osteoblasts.Results The transfected PMSCs still expressed stem cell markers such as CD29,CD44 and CD73.The mean cumulative time of population doubling was 23.9 hours.The cellular cycle retained the proliferative characterization of stem cells.Ultrastructural features of transfected P-MSCs included increased surface microvilli,abundant mitochondria and slightly swollen
多药耐药基因转染对人胎盘源性间充质干细胞生物学特性影响研究
