Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the P

Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva

prolifera

LI Jiaxin;ZHANG Pei;HONG Liang;LIU Chenguang

【期刊名称】《中国海洋大学学报（英文版）》 【年(卷),期】2019(018)001

【摘要】The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli.Furthermore,a

homogeneous

extracellular

polysaccharide

hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23％.Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 kDa and optimum activity at 45 ℃ and pH 6.0.The polysaccharide hydrolase maintained its stability within a wide range of pH (3.0-12.0) and thermal stability when the temperature was below 50C.The presence of Hg2+,Fe2+,Mn2+,Co2+ and SDS notably decreased hydrolase

activity,and

organic

solvents

such

as

formaldehyde,acetone,DMF and acetonitrile completely inhibited hydrolase activity.The purified hydrolase had no activity on agar,carrageenan,gellan gum,sodium alginate,or starch,but effectively

hydrolyzed the polysaccharide from Ulva prolifera.The Km and Vmax values of this hydrolase were 43.84mgmL-1 and 4.33 mgmL-1 min-1,respectively.The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase. 【总页数】8页(185-192) 【关键词】

【作者】LI Jiaxin;ZHANG Pei;HONG Liang;LIU Chenguang

【作者单位】College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_journal-ocean-university-china_thesis/0201270224455.html 【相关文献】

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