Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species

Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species

Maria A.Freitas;Antonia T.L.Santos;Antonio J.T.Machado;Ana Raquel P.Silva;Fábia F.Campina;Maria S.Costa;Gioconda M.A.B.Martins;Maria Flaviana B.Morais-Braga;Saulo R.Tintino;Irwin

R.A.Menezes;Jaime

Ribeiro-Filho;Altevir

P.Medeiros;Adeliana

S.Oliveira;Patrício B.Maracajá;Henrique D.M.Coutinho;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;

【期刊名称】《亚太热带生物医学杂志：英文版》 【年(卷),期】2017(000)011

【摘要】Objective: To investigate the antifungal activity of the fern species

Lygodium

venustum(L.

venustum)

and

Pityrogramma

calomelanos(P. calomelanos) against Candida albicans and Candida tropicalis strains.Methods: The microdilution method was used to evaluate the antifungal activity, as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole. The minimum

inhibitory

concentration(MIC),

minimum

fungicide

concentration and morphological changes were also determined.Results: The extract obtained from L. venustum presented a MIC > 8 192 mg/m L, while the extract obtained from and P. calomelanos presented a MIC = 8 192 mg/m L, indicating that they present weak antifungal activity. However, combination of the extracts with Fluconazole potentiated the antifungal activity of this drug. At different experimental conditions, such as concentration of the extract and type of strain, the extracts