利用大孔树脂制备灵芝活性三萜的研究
田振;唐庆九;冯娜;张劲松;王金艳
【期刊名称】《食用菌学报》 【年(卷),期】2016(023)004
【摘要】Fruiting bodies of Ganoderma lucidum were eluted by different concentration ethanol with D101 macroporous resin as filler.Different eluates
were
analyzed
by
HPLC
(High
Performance
Liquid
Chromatography) and screened their anti-tumor activities in vitro.Triterpenoids eluates obtained by elution of 90% and 95% ethanol with low-pole,total yield of 6.7%,shown the most strong inhibit activity to K562 and LNCaP (IC50 was 14.67 and 0.87 μg/mL,respectively).%用D101大孔树脂作为填料,不同浓度乙醇梯度洗脱灵芝(Ganoderma lucidum)子实体提取物,利用HPLC对得到的不同洗脱物进行了分析对比,并利用体外抗肿瘤模型对洗脱物进行了活性研究.结果表明,90%和95%乙醇洗脱获得了灵芝中极性较弱的三萜组分,得率共计为6.7%.该三萜组分具有较强的抗肿瘤活性,其中,以95%乙醇洗脱获得的三萜组分活性最强,对肿瘤细胞K562、LNCaP增殖抑制的IC50值分别达到14.67 μg/mL和0.87 μg/mL. 【总页数】5页(29-33)
【关键词】灵芝子实体;三萜;大孔树脂;抗肿瘤活性 【作者】田振;唐庆九;冯娜;张劲松;王金艳
【作者单位】上海海洋大学,上海201306;上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加