左归降糖解郁方对糖尿病并发抑郁症大鼠海马区星形胶质
细胞谷氨酸转运体的影响
徐雅岚;赵洪庆;杜青;杨蕙;孟盼;王宇红
【期刊名称】《北京中医药大学学报》 【年(卷),期】2016(039)006
【摘要】Objective To investigate the effects of Zuogui Jiangtang Jieyu Formulation(ZGJTJYF)on the expression of excitatory amino acid transporter 3 (EAAT 3 )and vesicular glutamate transporter 2 (VGLUT2)inhippocampalastrocytesofdiabetesmellitusratswithdepression(DD).Methods DD rats were induced by intragastrical administration of high-fat diet,tail intravenous injection of streptozoto-cin (STZ)with 28 days’chronic stress and were randomly divided into five groups—model group,posi-tive drug group (metformin with fluoxetine),high-,middle-,and low- dose of ZGJTJYF (high-dose, mid-dose,and low-dose)group.Healthy SD rats were assigned into blank control group.After four con-secutive weeks the cognition ability was measured using Morris water maze,and the expression of EAAT3 and VGLUT2 weredetected
by
using
immunohistochemical
double
labeling
method.Results Compared with blank control group,escape latency of model group was significantly longer (P<0.01 ),EAAT 3 expression decreased,VGLUT
2
expression
increased
remarkably
(both
P<0.05).Compared with mod-el group,escape latency of positive drug
左归降糖解郁方对糖尿病并发抑郁症大鼠海马区星形胶质细胞谷氨酸
