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IL-1β及其Ⅰ型受体在大鼠肺纤维化发生过程中的动态表达
作者：王小慧,刘学军
来源：《中国医药导报》2010年第09期
[摘要] 目的:观察大鼠肺纤维化发生中IL-1β及其Ⅰ型受体(IL-1RⅠ)的动态表达。方法:采用气管内注入博莱霉素制备肺纤维化模型,设正常组、模型组和甲强龙组,于第1、3、7、14、28天分批处死大鼠,观察各组大鼠各期病理切片,检测肺泡灌洗液及血清中IL-1β和其Ⅰ型受体的含量。结果:模型组早期肺泡炎症明显,后期肺组织实变。灌洗液及血清中IL-1β于7 d时达峰值,14 d时显著下降,28 d时回到正常水平,Ⅰ型受体的表达规律与IL-1β相同。结论:IL-1β及其Ⅰ型受体在肺纤维化早期呈现短暂的表达高峰,提示其可能在肺纤维化发生的早期起关键作用。
[关键词] 肺纤维化;IL-1β;IL-1RⅠ
[中图分类号] R-332[文献标识码]A [文章编号]1673-7210(2010)03(c)-019-02
Dynamic expression of IL-1β and IL-1RⅠ in the rats of pulmonary fibrosis WANG Xiaohui, LIU Xuejun*
(Department of Geriatrics, the First Hospital of Shanxi Medical University, Taiyuan 030001, China)
[Abstract] Objective: To investigate the changes of IL-1β and IL-1RⅠ in pulmonary fibrosis induced by bleomycin in rats. Methods: The pulmonary fibrosis model was established by intratracheal injection of bleomycin with rats, set the normal group,the model group and the
methylprednisolone group. The rats were killed on the 1st, 3rd, 7th, 14th and 28th day after treatment separately. The changes of biopsy of the lung tissue in every stage were investigated, the contents of IL-1β and IL-1RⅠ in BALF and blood serum were tested. Results: In the early period of pulmonary fibrosis, the inflammation in alveolar was obvious in the model group, while the lung tissue occurred consolidation at last. IL-1β in BALF and blood serum achieved peak on the 7th day, which was
decreased markedly on the 14th day, and down to the normal level almost on the 28th day. While the expression pattern of IL-1RⅠ was similar to that of IL-1β. Conclusion: IL-1β and IL-1RⅠ in
pulmonary fibrosis raise to the peak early and briefly, and play an important role in the earlier period of pulmonary fibrosis.
[Key words] Pulmonary fibrosis; IL-1β; IL-1RⅠ
IL-1β及其Ⅰ型受体在大鼠肺纤维化发生过程中的动态表达
