Negative association of donor age with CD34+ cell dose in mixture allografts of G-CSF-prim
Negative association of donor age with CD34+ cell dose in mixture allografts of G-CSF-primed bone marrow and G-CSF-mobilized peripheral blood
harvests
Li Yan;Chang Yingjun;Xu Lanping;Zhang Xiaohui;Huang Xiaojun
【期刊名称】《中华医学杂志(英文版)》 【年(卷),期】2014(127)020
【摘要】Background The effects of donor characteristics on CD34+ cell dose remain controversial.Recently,we developed a novel haploidentical transplant protocol,in which mixture allografts of granulocyte colony-stimulating factor (G-CSF)-primed bone marrow (G-BM) and G-CSF-mobilized peripheral blood (G-PB) were used.The aim of this study was to investigate the effects of donor characteristics on CD34+ cell dose in mixture allografts of G-BM and G-PB.Methods A total of 162 healthy adult donors,who underwent bone marrow harvest and peripheral blood collection between January 2009 and November 2010 in Peking University People's Hospital,were prospectively investigated.G-CSF was administered subcutaneously at a dose of 5 μg/kg once a day for 5-6 consecutive days.Bone marrow and peripheral blood stem cells were harvested on the fourth day and fifth day,respectively.A final total CD34+ cell dose less than 2× 106 cells/kg recipient body weight was considered a poor mobilization.Results Of the 162 donors,31 (19.1%)