Claudia Sch?fer, Global Customer Support, Penzberg ? 4486
MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys? 2010, cobas e 411
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Elecsys Dilution Recommendations
Reagent type Thyroid Function Test Test FT3 FT4 T3 T4 TSH T-uptake TG Anti-TG Anti-TPO Anti-TSHR Cardiac CK-MB (STAT) Digoxin Digitoxin Myoglobin (STAT) proBNP II (STAT only cobas e 601/2) Troponin I (STAT) Troponin T (STAT) Troponin T hs (STAT ) Recommended dilution ratios ? ? ? ? 1:10 with Diluent MA ? 1:5 with Diluent Universal @ @ 1:5 with Diluent Universal manually 1:5 or 1:10 with Anti-TSHR negative human sera* 1:2 with Diluent MA 1:2 with Diluent Universal 1:2 with Diluent Universal 1:10 with Diluent Universal 1:2 with Diluent Universal 1:10 with Diluent MA 1:10 with Diluent Universal 1:10 with Diluent MA Concentration of diluted sample must be > 10 μIU/ml > 50 ng/ml > 100 IU/ml > 4 IU/l > 50 ng/ml > 2.5 ng/ml >3.2 nmol/l > 30 ng/ml > 39 nmol/l > 50 ng/ml > 15000 pg/ml > 1770 pmol/l > 3 μg/l > 3 ng/ml > 1 μg/l > 1 ng/ml > 1000 ng/l
Autodilution possible for bold mentioned dilution ratios
? Dilution not necessary due to the broad measuring range or not possible e.g. can not be diluted because of changing in the concentration of the binding proteins alters this equilibrium.
@ The autoantibodies are heterogeneous and this gives rise to non-linear dilution phenomena. * Please check the package insert.
STAT for Elecsys 2010, cobas e 411, cobas e 601 (SW version 04-03 onwards) and cobas e 602 # Diluent Universal can be used to dilute the samples.
? Auto-dilution is not possible as assay uses a three-step method. ? Use Elecsys? Diluent Universal for automatic sample predilution. ? Autodilution not possible.
Diluent ratio Version 4.6 May 2011 1/7
Claudia Sch?fer, Global Customer Support, Penzberg ? 4486
MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys? 2010, cobas e 411
Elecsys? Dilution Recommendations Reagent type Hormones Test ACTH Cortisol DHEA-S Estradiol II FSH HCG+? HCG (STAT) LH Progesterone II Prolactin II SHBG Testosterone II C-Peptide (serum and urine) Insulin PlGF sFLT-1 Free ?hCG PAPP-A AFP CA 125 II CA 15-3 II ? Recommended dilution ratios ? 1:10 with Diluent Universal * (for urine samples) 1:5 with low concentrated human sera* 1:5 with Diluent MA ? 1:100 with Diluent Universal* ? 1:10 with Diluent Estradiol / Progesterone ? * 1:10 with Diluent Universal 1:10 with Diluent MA ? 1:10 with Diluent MA ? ? ?* 1:10 with Diluent Universal 1:10 with Diluent Universal 1:50 with Diluent Universal 1:5 with Diluent Universal 1:10 with Diluent Universal (only 1:10 possible on E170 / cobas e 601/2)
Concentration of diluted sample must be > 50 nmol/l > 1.8 μg/dl > 1.5 μmol/l > 45 μg/dl > 1835 pmol/l > 500 pg/ml > 100 mIU/ml > 6 nmol/l > 2 ng/ml > 50 μIU/ml, > 2.4 ng/ml > 20 nmol/l > 4 ng/ml > 1.3 nmol/l > 19 IU/l > 500 mIU/l > 20 U/ml, > 24 ng/ml > 1000 U/ml > 30 U/ml Diabetes Preeclampsia Down`s syndrome screening Tumor Marker CA 19-9 CA 72-4 CEA Cyfra 21-1 HE4 NSE Free PSA Total PSA S 100 Down`s syndrome screening Anaemia Free ?hCG PAPP-A Vitamin B12 1:10 with Diluent Universal 1:2 with Diluent Universal 1:50 with Diluent Universal 1:2 with Diluent Universal 1:20 with Diluent MA 1:2 with diluent NSE ? ? 1:50 with Diluent Universal 1:5 with S 100 analyt free human serum or CalSet 1 1:10 with Diluent Universal 1:10 with Diluent Universal 1:2? with Diluent Universal > 50 U/ml > 150 U/ml > 20 ng/ml > 250 ng/ml > 75 pmol/l > 50 ng/ml > 2 ng/ml > 1 μg/l > 19 IU/l > 500 mIU/l > 1000 pg/ml,
Diluent ratio Version 4.7 August 2011 2/7
Claudia Sch?fer, Global Customer Support, Penzberg ? 4486
MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys? 2010, cobas e 411
Elecsys? Dilution Recommendations Reagent type Test Recommended dilution ratios 1:50 with Diluent Universal 1:2? with Diluent Universal 1:2 with 0.2% ascorbic acid solution ? 1:2 with Diluent Universal 1:5 with Diluent Universal 1:2 with Diluent Universal ? 1:2 with Diluent Universal 1:2 with Diluent Universal 1:20 with Diluent Universal 1:2 to 1:5 with neg. human serum pool* 1:4 with neg. human sera 1:10 with Diluent MA with Diluent Hepatitis A ? # #* # #* #* 1:100 with Diluent Universal * 1:100 or 1:400 depending on the used system with Diluent HepB* * * * * 1:20 with Diluent Universal 1:20 with Diluent Universal* * * * 1:20 with Diluent Universal * 1:20 with Diluent Universal Concentration of diluted sample must be >738 pmol/l > 40 ng/ml > 10 ng/ml, > 22.7 nmol/l >16.2 nmol/l > 8 ng/ml > 25 ng/ml > 60 ng/ml > 100 μg/l > 1150 pg/nl > 122 pmol/l > 35 ng/ml > 87.5 nmol/l > 125 IU/ml, > 300 ng/ml > 1.0 ng/ml > 50 pg/ml > 20 IU/l > 10 IU/ml
Ferritin Folate III RBC Folate Bone ?-CrossLaps hGH N-MID Osteocalcin Total P1NP PTH (STAT) PTH (1-84) Vitamin D total Varia Rheumatoid Arthritis Sepsis Infectious Diseases IgE II Anti-CCP Brahms PCT IL 6 Anti-HAV Anti-HAV IgM ? Anti-HBc Anti-HBc IgM ? Anti-HBe HBeAg Anti-HBs HBsAg / HBsAg II HBsAg II quant Retroviruses Torch Anti-HCV HIV Ag HIV combi HIV combi PT CMV IgG CMV IgG Avidity CMV IgM? HSV-1 IgG HSV-2 IgG Rubella IgG Rubella IgM? Toxo IgG ? 15 U/ml > 10 IU/l > 3 IU/l
Diluent ratio Version 4.7 August 2011 3/7
Claudia Sch?fer, Global Customer Support, Penzberg ? 4486
MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys? 2010, cobas e 411
Elecsys? Dilution Recommendations Reagent type Test Recommended dilution ratios Concentration of diluted sample must be
Toxo IgM? #* Diluent ratio Version 4.7 August 2011
4/7
Claudia Sch?fer, Global Customer Support, Penzberg ? 4486
MODULAR ANALYTICS E170, cobas e 601, cobas e 602, Elecsys? 2010, cobas e 411
Elecsys? Dilution Recommendations
Used Documents:
Assay Package insert FT3 1109/12 FT4 0810/17 T3 0409/18 T4 0510/15 TSH 0510/19 T-uptake 0409/16 TG 0310/05 Anti-TG 0710/02 Anti-TPO 1010/03 Anti-TSHR 0610/08 CK-MB + STAT 0511/01 Myoglobin + STAT 0710/13 proBNP II 0510/05 Troponin I + STAT 1209/02 Troponin T + STAT 1110/06 Troponin T hs + STAT 0410/03 Digoxin 0610/15 Digitoxin 0310/09 HCG+? + HCG STAT 0310/14 ACTH 0810/05 Cortisol 0710/17 DHEA-S 0509/14 Estradiol II 0509/15 FSH 0509/16 LH 0509/16 Progesterone II 0810/13 Prolactin II 0509/03 SHBG 0509/08 Testosterone II 0710/04 C-Peptide 0810/05 Insulin 0810/10 PlGF 0810/03 sFLT-1 0710/03 AFP 0410/09 CA 125 II 1110/12 CA 15-3 II 0210/13 CA 19-9 0410/18 CA 72-4 0410/08 CEA 0410/18 Cyfra 21-1 0310/14 HE4 0111/01 NSE 0310/03 Diluent ratio Version 4.6
May 2011 5/7
罗氏电化学发光免疫分析仪项目推荐稀释比例
