一种新型锌指蛋白基因--人ZNF313的克隆、定位及表达
马用信;张思仲;侯一平;黄新力;吴洽庆;孙岩
【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2003(035)003
【摘要】A novel human zinc finger protein gene that contains both ring finger and C2H2 domain was first isolated by mRNA differential display between the testes of fertile adults and azoospermic patients followed by rapid amplification of cDNA ends (RACE). Total 6 exons of the human gene span a 17 484 bp genomic DNA sequence that was mapped to chromosome 20q13 by fluorescence in situ hybridization. The mature processed mRNA encodes a 228-amino acid protein with a C3HC4 ring finger and three C2H2 domains. Genomic analysis of the human gene identified two polyadenylation signals in exon 6 resulting in alternative 3′-untranslated regions. Results of Northern blot and RT-PCR of RNAs extracted from multiple tissues revealed that the gene has two transcripts of which the shorter transcript was expressed abundantly in fertile adult testes, but much less in testes of azoospermic patient, fetus as well as other human tissues. These data suggest that the gene may play a role in human spermatogenesis and male fertility.%运用正常可育男性和无精症病人睾丸组织的mRNA差异显示和cDNA末端快速扩增(RACE)等方法, 从人睾丸组织中分离了一个同时含有指环结构和C2H2结构域的新型锌指蛋白基因--人ZNF313.运用荧光原位杂交(FISH)方法, 将该基因定位到人染
一种新型锌指蛋白基因--人ZNF313的克隆、定位及表达
