Ⅱ型糖尿病大鼠颌骨骨髓基质干细胞体外培养及其成骨特
性观察
王俊成;王彬;鄂玲玲;郭靖;刘洪臣
【期刊名称】《中华老年口腔医学杂志》 【年(卷),期】2015(000)002
【摘要】目的：观察域型糖尿病大鼠颌骨骨髓基质干细胞(BMSCs)的成骨分化特性。方法：无菌条件下从10周龄GK大鼠(实验组)及同龄正常Wistar大鼠(对照组)下颌骨获取BMSCs，采用全骨髓贴壁培养法对细胞进行纯化及培养，流式细胞仪检测干细胞表型.取第3代细胞成骨诱导培养，分别对两组细胞行茜素红染色，碱性磷酸酶染色及活性测定，培养基钙、磷含量测定，以及real-time PCR测定Runx2基因表达变化。结果：成功培养出域型糖尿病大鼠颌骨来源的BMSCs，与正常组比较，实验组BMSCs茜素红染色变浅、钙结节形成减少，碱性磷酸酶(ALP)活性下降，细胞钙、磷分泌显著降低，差异有统计学意(P＜0.05)；Runx2 mRNA表达也明显降低(P＜0.05)。结论：域型糖尿病大鼠颌骨来源的BMSCs的成骨分化能力受到损害，并且即使在正常糖浓度下培养其成骨能力仍无法恢复。 jective:To observe osteogenic differentiation ability of bone marrow stromal cells (BMSC) obtained from type 2 diabetic rats mandibles.Methods:The invitrotechnique of cell culture was employed to harvest, purify and cultivate BMSCs from the mandibles of type 2 diabetic(GK, test group) and normal rats (Wistar,control group) . Flow cytometry analyses of cell phenotype and osteogenic/adipogenic differentiation were performed. The passage 3 cells were incubated with
Ⅱ型糖尿病大鼠颌骨骨髓基质干细胞体外培养及其成骨特性观察
