双波长比值光谱法测定银黄口服液中绿原酸和黄芩苷含量

双波长比值光谱法测定银黄口服液中绿原酸和黄芩苷含量

孙增先;李永溟;李瑞真;王奎兴;张骞峰

【期刊名称】《中国现代应用药学》 【年(卷),期】2001(018)001

【摘要】OBJECTIVE：To determine of chlorogenic acid and baicalin in Yinhuang

oral

solution

by

dual-wavelength

ration

spectrometry.METHODS:With the feature of ratio spectrometry for chlorogenic acid and baicalin,266 and 342nm were chosen as their determination wavelengthes.RESULTS:Linear range of chlorogenic acid and baicalin were 2～20μg/ml and 2～10μg/ml respectively.The recovery of chlorogenic acid and baicalin rate were 98.9%(RSD=1.58%) and 100.4%(RSD=1.32%) respectively.CONCLUSION:As a precise,accute and sample method,it could be spread to the assay of other traditional Chinese medicine products.%目的：应用双波长比值光谱法对银黄口服液中绿原酸和黄芩苷含量测定进行研究。方法：依据绿原酸和黄芩苷的比值光谱特征，选择266和342nm作为测定波长。结果：绿原酸线性范围2～20μg/ml,回收率98.9,RSD为1.58%;黄芩苷线性范围2～16μg/ml,回收率100.4%,RSD为1.32%。结论：本法为一种灵敏、准确和简便的分析方法，可在其他中药制剂中推广应用。 【总页数】3页(55-57)

【关键词】双波长比值光谱法;银黄口服液;绿原酸;黄芩苷 【作者】孙增先;李永溟;李瑞真;王奎兴;张骞峰