甲型流感病毒H9N2亚型毒株血凝素基因特性的研究
郭元吉;董婕;王敏;张烨;郭俊峰;吴昆昱
【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2001(114)001
【摘要】Objective To determine the origin of human influenza A (H9N2) virus and the relationship among H9N2 strains isolated from different hosts, on the basis of molecular biology. rnMethods Viruses were passed in embryonated hen eggs, and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit. Afterwards RNA sequence analysis was performed by dideoxynucleotide chain termination and a cloning method. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (version 1.03) and Editseg (version 3.69) softwares.rnResults The amino acid sequences at the cleavage site between HA1 and HA2 domains of H9N2 viruses isolated in China are R-S-S-R. One pigeon strain contains seven potential glycosylation sites on the HA protein molecule, while all others have eight. There are 2 to 15 differences of amino acid sequences distributed at 24 different positions on the HA protein molecules among six H9N2 viruses. The H9N2 viruses with multiple lineages of HA genes were co-circulating in China recently.rnConclusion The highest possibility is that human influenza A
(H9N2) virus was derived from Chicken H9N2 virus, and not derived from pigeon H9N2 virus. However, it is still unknown whether the H9N2 virus could transmit from person to person. The H9N2 viruses with multiple lineages of HA genes are co-circulating in China.%目的了解人甲型流感病毒H9N2亚型毒株的来源及从分子生物学角度来弄清不同宿主来源的H9N2亚型毒株间相互关系。rn方法病毒在鸡胚中传代，从收获的尿囊液中提取病毒粒RNA，通过逆转录合成cDNA。cDNA通过PCR进行扩增，接着PCR产物用纯化试剂盒进行纯化。而后，RNA序列测定是采用双脱氧链末端终止和克隆法。最后用MegAlign（1.03版）和Editseq（3.69版）软件进行种系发生学分析。rn结果从中国所分离出的H9N2亚型毒株。它们的HA1与HA2间裂解部位的氨基酸序列均为R-S-S-R，除一株鸽病毒其HA蛋白分子上仅含7个潜在的糖基化位点外,而其余的均含8个。6株H9N2毒株HA蛋白分子上氨基酸序列有2-16个不同，这些不同分布在24个不同的位点上。近来在中国同时流行着多种HA基因系的H9N2亚型毒株。rn结论人甲型流感病毒H9N2亚型毒株可能性最大来自鸡的H9N2毒株，而不是来源于鸽的H9N2毒株。但，H9N2毒株是否具有人传人能力，至今仍不清楚。在中国同时流行着多种HA基因系的H9N2毒株。 【总页数】4页(76-79)
【关键词】甲型流感病毒 H9N2亚型 HA基因特性 【作者】郭元吉;董婕;王敏;张烨;郭俊峰;吴昆昱
【作者单位】中国预防医学科学院病毒学研究所流感研究室北京 100052;中国预防医学科学院病毒学研究所流感研究室北京 100052;中国预防医学科学院病
毒学研究所流感研究室北京 100052;中国预防医学科学院病毒学研究所流感研究室北京 100052;中国预防医学科学院病毒学研究所流感研究室北京 100052;中国预防医学科学院病毒学研究所流感研究室北京 100052 【正文语种】中文 【中图分类】R511 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-medical-journal_thesis/0201255008342.html 【相关文献】
1.甲型流感病毒(H1N2)亚型毒株血凝素和神经氨酸酶基因来源的研究 [J], 祁俊林; 王敏; 董婕
2.一株从死亡黑天鹅中分离出H5N1亚型流感病毒株血凝素基因特性的研究 [J], 程小雯; 吕星; 房师松; 刘建军; 周丽; 何建凡
3.鸭流感病毒4/2004-H6N2亚型毒株的血凝素基因全序列克隆与分析 [J], 顾节清; 陈杰; 李晓成; 杨增岐; 吴发兴; 张燕霞; 黄保续
4.贵州省3株甲3(H3N2)亚型流感病毒的血凝素基因HA1区序列分析 [J], 庄丽; 庄妍; 阎岩; 胡静; 付琳
5.甲1亚型流感病毒变异株A/河北／44／97血凝素HAl基因的核苷酸序列分析 [C],
以上内容为文献基本信息，获取文献全文请下载
甲型流感病毒H9N2亚型毒株血凝素基因特性的研究
