约氏疟原虫Pys48核酸疫苗免疫效应的观察
单颖;赵忠毅;刘英杰;郑丽;刘军;延娟;曹雅明
【期刊名称】《中国人兽共患病学报》 【年(卷),期】2012(028)005
【摘要】To evaluate the characteristics of immune function after immunization by P. Yoelii 17XL Pys48 DNA plasmid, the BALB/c mice were intramuscularly injected with Pys48 DNA plasmid and empty plasmid as control. The mice were sacrificed after the 3rd cycle of immunization. The levels of IFN-y,IL-4 in splenocyte supernatant obtained from each group mice were assayed by ELISA. Flow cytometry was applied to quantitatively analyzed subset of DCs and DCs expressing TLR9, and B cells secreting IgG. It was found that IL-4 level from Pys48 DNA vaccine immunized mice increased obviously, compared to that from empty plasmid and normal control group (P<0. 05). And there was no obvious difference in IFN-y level between each group. The number of splenocyte mDCs had no apparent disparity, while pDCs number from vaccine immunized mice increased significantly. Moreover, the percentage contents of DCs expressing TLR9 and B cells secreting IgG were higher than those of other two control groups (P<0. 05). These results indicated that Pys48 DNA plasmid possessed a pretty immunogenicity and could develop vaccine effect through strengthening humoral immunity.%目的 探讨约氏疟原虫
约氏疟原虫Pys48核酸疫苗免疫效应的观察
