Current Medical ScienceDOI https://doi.org/10.1007/s 11596-019-2035J305Expression Patterns and Implications of LaminBl in Rat CochleaeZhi-hui DU, Jin CHEN, Qing-guo CHEN, Liang-qiang ZHOU, Dan BING, Yun LIU, Yan-bo SUN, Peng-jun LI, Fan QI, Hong-mei ZHU, Han-qi CHU#Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China? Huazhong University of Science and Technology 2019Summary: LaminBl, a major component of the nuclear lamina, is a potent regulator of cellular proliferation and senescence and also known to be essential for neuronal migration and brain development. However, the expression patterns of LaminB 1 in the rat cochleae are still not fully revealed. Utilizing immunofluorescence. Western blotting, and quantitative real-time PCR, we identified the distribution and expression of LaminB 1 in the rat cochleae. Immunofluorescence staining indicated that LaminBl was mainly localized in the auditory hair cells (HCs), spiral ganglion cells (SGC), stria vascularis (STV, including spiral ligament), Reissner's membrane (RM), and limbus laminae spiralis (LLS). Western blotting analysis illustrated that the distribution of LaminBl in rat cochleae was characterized by tissue specificity. The LaminB 1 protein was expressed more in SGC and basilar membrane (BM) than in STV. Meanwhile, the mRNA expression of LaminB 1 displayed difference in cochlear tissues. These observations preliminarily revealed the expression patterns of LaminB 1, providing a theoretical basis for further study on the role of LaminBl in auditory function.Key words: LaminBl; cochleae; rat; hair cell; spiral ganglion cell; stria vascularisSensorineural hearing loss (SNHL) is the most common form of hearing loss at present11]. The loss of peripheral tissue or cell death in the cochlea is the typical cause of SNHL[2]. In the cochlea, especially in the scala media, many interconnected cell types are essential for hearing, such as the hair cells and the supporting cells. The auditory sensory epithelium hair cells transmit the signal of mechanical stimuli to the spiral ganglion neurons (SGNs) through their dendrites. Then the information will be carried to the brain by the axon⑶.Many studies have focused on the biochemical, molecular, and intracellular mechanisms in normal-state hearing function and in pathological processes that impact the viability of cochlear sensorineural tissues[2].The nuclear lamina is an intermediate filament meshwork, located in the inner membrane of the nuclear envelope and involved in the maintenance of the physiological equilibrium of cells[4]. The major structural proteins of the lamina are divided into A- and B?types⑸.The two similar but functionally distinctZhi-hui DU, E-mail: zhihui32422@ 163.com
^Corresponding author. E-mail: qi7chu@163.com*This project was supported by the National Natural Science Foundation of China (No. 81771004, No. 81500791, and No. 81300827).isoforms of vertebrate A-type lamins, Lamin A and C, are the major products of alternative splicing of LMNA[6\\ Lamin Bl and Lamin B2 are the two major B-type lamins in most vertebrates. They are encoded by the LMNB1 and LMNB2 genes, respectively171. At least one B-type lamina continues to be expressed in all cells throughout development181.LaminB 1 is thought to be essential for chromatin organization, DNA replication and regulation of gene expression19, l0]. LaminB 1 also has a crucial role in the assembly of the mitotic spindle, and that the spindle complex is disrupted by a dominant negative LaminB 1 mutant[11]. In addition, LaminB 1 seems to play a specific role in the proliferation, survival and differentiation of certain tissues and cell types1121, especially in the nervous system. Specifically, a duplication of the human LaminBl gene {LMNB1} is thought to cause adult-onset autosomal dominant leukodystrophy (ADLD)[I3], a kind of laminopathy affecting myelination of the central nervous system (CNS). The deficiency of LMN Bl results in perinatal lethality, reduced brain size, disorganized layering and apoptosis of cortical neurons during embryonic corticogenesis[14]. Meanwhile, LMNB1 knockout mice exhibit abnonnal lung development and bone ossification during embiyogenesis and die shortly after
Expression Patterns and Implications of LaminBl in Rat Cochleae - 论文
