Influence of various stressors on the expression of core genes of the small interfering RN

Influence of various stressors on the expression of core genes of the small interfering RNA pathway in

the oriental fruit fly, Bactrocera dorsalis

Yi-Fei Xie;Jin-Zhi Niu;Xuan-Zhao Jiang;Wen-Jia Yang;Guang-Mao Shen;Dong Wei;Guy Smagghe;Jin-Jun Wang

【期刊名称】《中国昆虫科学：英文版》 【年(卷),期】2017(024)003

【摘要】RNA interference (RNAi)-based technology has emerged as a potential tool for controlling insect pests,however,previous studies found that the efficiency of RNAi in Bactrocera dorsalis was variable.In nature,insects often meet various challenges,such as pathogen infections,extreme temperatures,lack of nutrition and heavy metals.To better understand the association of the stressors with efficiency of RNAi,in the current study we tested the expression of three core genes,dicer2 (Bddcr2),r2d2 (Bdr2d2) and argonaute2 (Bdago2),of the small interfering RNA (siRNA) pathway of B.dorsalis upon various stressors.Our results showed that all three genes were upregulated by the infection of invertebrate iridescent virus 6,which suggested a function of the siRNA pathway against viral infection.The loading of FeCl3 could also increase the expression of Bddcr2.The treatments ofEscherichia coli,extremely high (40℃) and low (0℃) temperatures,as well as starvation,could negatively influence the expression of Bddcr2