PSD95 Gene Specific siRNAs Attenuate Neuropathic Pain through Modulating Neuron Sensibilit

PSD95 Gene Specific siRNAs Attenuate Neuropathic Pain through Modulating Neuron

Sensibility and Postsynaptic CaMKⅡα

Phosphorylation

Le Shen;Xu Li;Nen Chen;Li Xu;Wei Liu;Xue-rong Yu;Yu-guang Huang

【期刊名称】《中国医学科学杂志（英文版）》 【年(卷),期】2011(026)004

【摘要】Objective To observe the effects of PSD95 gene specific siRNAs on

neuropathic

pain

relief,neuron

protein

viability,and kinase

Ⅱα

postsynaptic (CaMKⅡα)

calcium/calmodulin-dependent

phosphorylation in vitro and in vivo.Methods Gene-specific siRNAs of rat PSD95 were synthesized chemically for transfection.Adult male Sprague-Dawley (SD) rats were randomly divided into 3 groups:naive group (n=6),sham group (n=6),and sciatic nerve chronic constriction injury (CCI) group (n=24).The CCI group was further divided into 4 groups (n=6 in each group),which were pretreated with normal saline,transfection vehicle,negative control siRNAs,and PSD95 gene specific siRNAs respectively.All the subgroups received corresponding agents intrathecally for 3 days,started one day before the CCI of sciatic nerve.Both mechanical allodynia and thermal hyperalgesia were measured on post-operative day 3 and 7.PSD95 gene silenced NG108-15 cells were further stimulated by glutamate,with the cell viability and