绿盲蝽丝氨酸蛋白酶基因AlSP4的克隆及取食不同寄主植
物后的表达谱分析
孙洋;柏立新;张永军;肖留斌;谭永安;吴国强
【期刊名称】《昆虫学报》 【年(卷),期】2012(055)006
【摘要】Some serine proteases, such as trypsin and chymotrypsin, are important digestive enzymes in the digestive system of mind bugs. In order to better understand the role of serine proteases in the digestive system of the green plant bug, Apolygus lucorum ( Meyer-Dllr) , we cloned the cDNA encoding serine protease of A. lucorum for the first time in the laboratory, which was named as AlSPA ( GenBank accession no. JQ609682). The results of sequence analysis showed that the open reading frame (ORF) of AlSP4 is 999 bp in length, encoding a 332-amino-acid peptide, with the predicted molecular weight (MW) of 36. 84 kDa and the theoretical isoelectric point (pl) of 5.35, and the predicted N-terminal hydrophobic region containing 16 amino acid residues displays the typical feature of a signal peptide. Protein signature analysis revealed that the protein encoded by AlSP4 shares typical structural features of serine proteases with other insects, including His, Asp, and Ser residues for the catalytic amino acid triad of active sites of serine proteases. Putative trypsin precursors from the encoded protein of AISP4 cDNA contain a signal peptide, activation
绿盲蝽丝氨酸蛋白酶基因AlSP4的克隆及取食不同寄主植物后的表达谱分析
